Key features and details
- Rabbit polyclonal to Bad (phospho S155)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Bad (phospho S155) antibody
See all Bad primary antibodies
DescriptionRabbit polyclonal to Bad (phospho S155)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human Bad around the phosphorylation site of Serine 155.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab28825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionPromotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Tissue specificityExpressed in a wide variety of tissues.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
modificationsPhosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
- Information by UniProt
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
Ab28825, at a concentration of 1/50, staining Bad in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: untreated.
Right image: treated with synthesized phosphopeptide.
Anti-Bad (phospho S155) antibody (ab28825) at 1/500 dilution + extracts from 293 cells (5-30ug) treated with forsklin
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
ab28825 has been referenced in 3 publications.
- Boac BM et al. Expression of the BAD pathway is a marker of triple-negative status and poor outcome. Sci Rep 9:17496 (2019). PubMed: 31767884
- Zhao L et al. Protein kinase A determines platelet life span and survival by regulating apoptosis. J Clin Invest 127:4338-4351 (2017). PubMed: 29083324
- Danial NN et al. Dual role of proapoptotic BAD in insulin secretion and beta cell survival. Nat Med 14:144-53 (2008). PubMed: 18223655