Anti-Bad (phospho S155) antibody (ab28825)
Key features and details
- Rabbit polyclonal to Bad (phospho S155)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Bad (phospho S155) antibody
See all Bad primary antibodies -
Description
Rabbit polyclonal to Bad (phospho S155) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Bad (phospho S155).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
|
IHC-P |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
-
Function
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. -
Tissue specificity
Expressed in a wide variety of tissues. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Post-translational
modificationsPhosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. -
Cellular localization
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 572 Human
- Omim: 603167 Human
- SwissProt: Q92934 Human
- Unigene: 370254 Human
-
Alternative names
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
see all
Images
-
Ab28825, at a concentration of 1/50, staining Bad in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: untreated.
Right image: treated with synthesized phosphopeptide. -
Anti-Bad (phospho S155) antibody (ab28825) at 1/500 dilution + extracts from 293 cells (5-30ug) treated with forsklin
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab28825 has been referenced in 5 publications.
- Boac BM et al. Expression of the BAD pathway is a marker of triple-negative status and poor outcome. Sci Rep 9:17496 (2019). PubMed: 31767884
- Ke J et al. Edaravone reduces oxidative stress and intestinal cell apoptosis after burn through up-regulating miR-320 expression. Mol Med 25:54 (2019). PubMed: 31829167
- Zhao L et al. Protein kinase A determines platelet life span and survival by regulating apoptosis. J Clin Invest 127:4338-4351 (2017). PubMed: 29083324
- Rong F et al. Knockdown of RhoGDIα induces apoptosis and increases lung cancer cell chemosensitivity to paclitaxel. Neoplasma 59:541-50 (2012). PubMed: 22668020
- Danial NN et al. Dual role of proapoptotic BAD in insulin secretion and beta cell survival. Nat Med 14:144-53 (2008). PubMed: 18223655