Western blot abreview for Anti-Bad (phospho S155) antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (12% acrylamide)
Loading amount
45 µg
Specification
Heart
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/40000
Name
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: ~23 kDa

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Submitted Jul 22 2015

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