Anti-BAF170 antibody (ab238120)
Key features and details
- Rabbit polyclonal to BAF170
- Suitable for: IHC-P, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BAF170 antibody
See all BAF170 primary antibodies -
Description
Rabbit polyclonal to BAF170 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BAF170 aa 300-650.
Database link: Q8TAQ2 -
Positive control
- ChIP: HeLa IP. IHC-P: Human epityphlon and colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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ChIP |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/20 - 1/200. |
ChIP
Use at an assay dependent concentration. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6601 Human
- Omim: 601734 Human
- SwissProt: Q8TAQ2 Human
- Unigene: 236030 Human
- Unigene: 632717 Human
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Alternative names
- BAF 170 antibody
- BAF170 antibody
- BRG1 associated factor 170 antibody
see all
Images
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Paraffin-embedded human colon cancer tissue stained for BAF170 with ab238120 at a 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human epityphlon tissue stained for BAF170 with ab238120 at a 1/100 dilution in immunohistochemical analysis.
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Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (1.1 x 106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 µg ab238120 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238120 has not yet been referenced specifically in any publications.