Key features and details
- Rabbit polyclonal to BAF170
- Suitable for: IHC-P, ChIP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BAF170 antibody
See all BAF170 primary antibodies
DescriptionRabbit polyclonal to BAF170
Tested applicationsSuitable for: IHC-P, ChIPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human BAF170 aa 300-650.
Database link: Q8TAQ2
- ChIP: HeLa IP. IHC-P: Human epityphlon and colon cancer tissue.
Protein previously labeled as SMRC2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab238120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ChIP||Use at an assay dependent concentration.|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain.
- Information by UniProt
- BAF 170 antibody
- BAF170 antibody
- BRG1 associated factor 170 antibody
Paraffin-embedded human colon cancer tissue stained for BAF170 with ab238120 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human epityphlon tissue stained for BAF170 with ab238120 at a 1/100 dilution in immunohistochemical analysis.
Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (1.1 x 106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 µg ab238120 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238120 has not yet been referenced specifically in any publications.