Overview

  • Product name

  • Description

    Rabbit polyclonal to BAF170
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ChIPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human BAF170 aa 300-650.
    Database link: Q8TAQ2

  • Positive control

    • ChIP: HeLa IP. IHC-P: Human epityphlon and colon cancer tissue.
  • General notes

    Protein previously labeled as SMRC2.

Properties

Applications

Our Abpromise guarantee covers the use of ab238120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
ChIP Use at an assay dependent concentration.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAF 170 antibody
    • BAF170 antibody
    • BRG1 associated factor 170 antibody
    • BRG1-associated factor 170 antibody
    • Chromatin remodeling complex BAF170 subunit antibody
    • CRACC 2 antibody
    • CRACC2 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 170 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARCC 2 antibody
    • Smarcc2 antibody
    • SMRC2_HUMAN antibody
    • SWI/SNF complex 170 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 2 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2 antibody
    • SWI3 like protein antibody
    see all

Images

  • Paraffin-embedded human colon cancer tissue stained for BAF170 with ab238120 at a 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human epityphlon tissue stained for BAF170 with ab238120 at a 1/100 dilution in immunohistochemical analysis.

  • Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (1.1 x 106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 µg ab238120 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.

References

ab238120 has not yet been referenced specifically in any publications.

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