Key features and details
- Rabbit polyclonal to BAF170
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BAF170 antibody
See all BAF170 primary antibodies
DescriptionRabbit polyclonal to BAF170
Specificityab64853 detects endogenous levels of total SMRC2 / BAF170 protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human BAF170 (internal sequence).
- Extracts from HUVEC cells and COLO205 cells.
Protein previously labeled as SMRC2.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 133 kDa).|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the SMARCC family.
Contains 1 SANT domain.
Contains 1 SWIRM domain.
- Information by UniProt
- BAF 170 antibody
- BAF170 antibody
- BRG1 associated factor 170 antibody
All lanes : Anti-BAF170 antibody (ab64853) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from COLO205 cells
Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
ab64853 has not yet been referenced specifically in any publications.