Overview

  • Product name

  • Description

    Rabbit polyclonal to BAF170
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human BAF170 aa 1164-1214 (C terminal). Synthetic peptide corresponding to a region between amino acid 1164 and the C terminus (amino acid 1214) of human BAF170 (NP_003066.2).

  • Positive control

    • Human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Colon Carcinoma, Laryngeal Squamous Cell Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Metastatic Lymph Node, Non Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma and Testicular Seminoma.
      Mouse Control: Squamous Cell Carcinoma.
  • General notes

    Protein previously labeled as SMRC2.

Properties

Applications

Our Abpromise guarantee covers the use of ab84453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the SMARCC family.
      Contains 1 SANT domain.
      Contains 1 SWIRM domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAF 170 antibody
      • BAF170 antibody
      • BRG1 associated factor 170 antibody
      • BRG1-associated factor 170 antibody
      • Chromatin remodeling complex BAF170 subunit antibody
      • CRACC 2 antibody
      • CRACC2 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 170 antibody
      • Rsc 8 antibody
      • Rsc8 antibody
      • SMARCC 2 antibody
      • Smarcc2 antibody
      • SMRC2_HUMAN antibody
      • SWI/SNF complex 170 kDa subunit antibody
      • SWI/SNF complex subunit SMARCC2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin c2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 2 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2 antibody
      • SWI3 like protein antibody
      see all

    Images

    • ab84453, at a 1/500 dilution, staining BAF170 in formalin fixed, paraffin embedded human prostate adenocarcinoma by Immunohistochemistry.
      Detection: using DAB staining.

    • ab84453, at 1/500 dilution, staining BAF170 in formalin fixed, paraffin embedded mouse squamous cell carcinoma by Immunohistochemistry.
      Detection: using DAB staining.

    References

    This product has been referenced in:

    • Cabot B  et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). Read more (PubMed: 29024220) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Gracias por contactarnos.

    He chequeado la homología de dichos anticuerpos con BAF155, y el resultado es el siguiente:

    ab64853: Al llevar a cabo un alineamiento de secuencias con BAFF15 (http://www.uniprot.org/uniprot/Q92922) resulta un porcentaje del 54.5% entre ambas secuencias.

    Ab71907: no presenta ninguna homología con la proteína BAFF15. Esta testado (y por tanto garantizado durante 6 meses) en WB e ICC/IF con células humanas.

    ab84453: el inmunógeno corresponde a la secuencia entre los aminoácidos 1164 y el extremo C terminal (aa 1214) de la proteína SMRC2 humana.

    ab135121: se ha generado a partir de un péptido correspondiente a los aminoácidos 1060-1130 de la proteína SMARCC2 humana (uniprot# Q8TAQ2). Comparte pocos residuos (alrededor de 16%) con BAF155.

    Si necesitas alguna otra aclaración, presupuesto o tienes cualquier otra consulta, por favor no dudes en contactarnos.

    Te recuerdo que todos nuestros productos tienen una garantía (Abpromise) de 6 meses, y en caso de no funcionar como se especifica en su datasheet, se os reemplazará el producto de forma gratuita o se reembolsará el importe del mismo.

    Read More

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