Recombinant

Recombinant Anti-Baf180 antibody [BL-39-2C3] (ab243876)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-39-2C3] to Baf180
  • Suitable for: WB, IHC-P, ICC, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Baf180 antibody [BL-39-2C3]
    See all Baf180 primary antibodies

  • Description

    Rabbit monoclonal [BL-39-2C3] to Baf180

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Baf180 aa 1-293.
    Database link: Q86U86

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab243876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation.

  • Tissue specificity

    Widely expressed.

  • Involvement in disease

    Defects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.

  • Sequence similarities

    Contains 2 BAH domains.
    Contains 6 bromo domains.
    Contains 1 HMG box DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • BAF180 antibody
      • BRG1-associated factor 180 antibody
      • CG11375 antibody
      • hPB1 antibody
      • MGC156155 antibody
      • MGC156156 antibody
      • OTTHUMP00000202149 antibody
      • OTTHUMP00000202150 antibody
      • OTTHUMP00000202151 antibody
      • OTTHUMP00000202153 antibody
      • OTTHUMP00000202163 antibody
      • PB1 antibody
      • PB1_HUMAN antibody
      • Pbrm1 antibody
      • polybromo 1 antibody
      • Polybromo-1D antibody
      • Protein polybromo-1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Baf180 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Formalin-fixed, paraffin-embedded human Breast tissue stained for Baf180 using ab243876 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
    • Immunoprecipitation - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Immunoprecipitation - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Baf180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution.

      Lane 1: ab243876 IP in HEK-293T whole cell lysate.

      Lane 2: Contol IgG in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 3 minutes.

    • Western blot - Anti-Baf180 antibody [BL-39-2C3] (ab243876)
      Western blot - Anti-Baf180 antibody [BL-39-2C3] (ab243876)

      Western blot analysis using ab243876 at 1/1000 dilution.

      Lane 1: HeLa whole cell lysate (15 µg).

      Lane 2: HEK-293T whole cell lysate (15 µg).

      Lane 3: MCF7 whole cell lysate (15 µg).

      Lane 4: HepG2 whole cell lysate (15 µg).

      Lane 5: A549 whole cell lysate (15 µg).

      Lane 6: SW620 whole cell lysate (15 µg).

      Lane 7: SK whole cell lysate (15µg).

      Lane 8: Jurkat whole cell lysate (15 µg).

      Lane 9: TCMK-1 whole cell lysate (15 µg).

      A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab243876? Please let us know so that we can cite the reference in this datasheet.

    ab243876 has not yet been referenced specifically in any publications.

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