Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-39-2C3] to Baf180
- Suitable for: WB, IHC-P, ICC, IP
- Reacts with: Human
Product nameAnti-Baf180 antibody [BL-39-2C3]
See all Baf180 primary antibodies
DescriptionRabbit monoclonal [BL-39-2C3] to Baf180
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Baf180 aa 1-293.
Database link: Q86U86
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Sequence similaritiesContains 2 BAH domains.
Contains 6 bromo domains.
Contains 1 HMG box DNA-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BAF180 antibody
- BRG1-associated factor 180 antibody
- CG11375 antibody
Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Baf180 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Formalin-fixed, paraffin-embedded human Breast tissue stained for Baf180 using ab243876 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Baf180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution.
Lane 1: ab243876 IP in HEK-293T whole cell lysate.
Lane 2: Contol IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot analysis using ab243876 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane 3: MCF7 whole cell lysate (15 µg).
Lane 4: HepG2 whole cell lysate (15 µg).
Lane 5: A549 whole cell lysate (15 µg).
Lane 6: SW620 whole cell lysate (15 µg).
Lane 7: SK whole cell lysate (15µg).
Lane 8: Jurkat whole cell lysate (15 µg).
Lane 9: TCMK-1 whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243876 has not yet been referenced specifically in any publications.