Key features and details
- Mouse monoclonal [CL0331] to Baf180
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Baf180 antibody [CL0331]
See all Baf180 primary antibodies
DescriptionMouse monoclonal [CL0331] to Baf180
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human endometrium, kidney and breast cancer tissue. WB: Human RT-4 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab242318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Sequence similaritiesContains 2 BAH domains.
Contains 6 bromo domains.
Contains 1 HMG box DNA-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BAF180 antibody
- BRG1-associated factor 180 antibody
- CG11375 antibody
Paraffin-embedded human endometrium tissue stained for Baf180 using ab242318 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-Baf180 antibody [CL0331] (ab242318) at 1 µg/ml
Lane 1 : RT-4 cells transfected with control siRNA
Lane 2 : RT-4 cells transfected with target specific siRNA probe
Paraffin-embedded human breast cancer tissue stained for Baf180 using ab242318 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for Baf180 using ab242318 at 1/50 dilution in immunohistochemical analysis.
Anti-Baf180 antibody [CL0331] (ab242318) at 1 µg/ml + Human RT-4 whole cell lysate
Paraffin-embedded human kidney cancer tissue stained for Baf180 using ab242318 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242318 has not yet been referenced specifically in any publications.