• Product name
    Anti-BAF53A antibody - ChIP Grade
    See all BAF53A primary antibodies
  • Description
    Rabbit polyclonal to BAF53A - ChIP Grade
  • Host species
  • Specificity
    In Western blot, ab3882 detects BAF53 and Tip60-TAP in total extracts of Tip60-TAP. Following TEV elution of Tip60-TAP ab3882 only detects BAF53.
  • Tested applications
    Suitable for: ChIP, ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A.

    Read Abcam's proprietary immunogen policy (Peptide available as ab13770.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) This antibody gave a positive signal in the following tissue lysates: Skeletal Muscle (Mouse) Ovary (Mouse) - normal tissue



Our Abpromise guarantee covers the use of ab3882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 53 kDa.
IHC-P 1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/400.


  • Relevance
    This protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • Cellular localization
  • Database links
  • Alternative names
    • 53 kDa BRG1 associated factor A antibody
    • Actin like 6A antibody
    • Actin like protein 6A antibody
    • Actin related protein antibody
    • Actin related protein Baf53a antibody
    • actin-related protein 4 antibody
    • ACTL 6 antibody
    • ACTL 6A antibody
    • ACTL6 antibody
    • ACTL6A antibody
    • Arp4 antibody
    • ARPN BETA antibody
    • ArpNbeta antibody
    • BAF 53 antibody
    • BAF 53A antibody
    • BAF complex 53 kDa subunit antibody
    • BAF53 antibody
    • BRG1 associated factor 53A antibody
    • BRG1 associated factor antibody
    • BRG1/brm associated factor 53A antibody
    • hArpN beta antibody
    • INO80 complex subunit K antibody
    • INO80K antibody
    • MGC5382 antibody
    see all


  • All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1/1000 dilution

    Lane 1 : TEV elution of Tip60-TAP clone 1

    Lane 2 : TEV elution of Tip60-TAP clone 2
    Lane 3 : Total extract of Tip60-TAP clone 1
    Lane 4 : Total extract of Tip60-TAP clone 2

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Total extracts from two different clones of MCF7 cells expressing a TAP tagged version of Tip60 were partially purified over IgG sepharose resin and eluted with TEV protease.  Western blot was performed using a 1/1000 dilution of the BAF53A antibody.

    Lane 1. TEV elution of Tip60-TAP clone #1
    Lane 2. TEV elution of Tip60-TAP clone #2
    Lane 3. Total extract of Tip60-TAP clone #1
    Lane 4. Total extract of Tip60-TAP clone #2

    NB: Tip60 is detected in the total extract due to the presence of Protein A in the TAP Tag (to which the primary and secondary abs bind). The TEV protease removes the Tag in lanes 1 and 2.

  • Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3882 to BAF53A and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 8 µl of ab3882 and 2x106 cells were used in each ChIP experiment.

  • ab3882 at a 1/200 dilution staining asynchronous HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and incubated with the antibody for 30 minutes at room temperature. Bound antibody was detected using a Cy3 conjugated Goat anti-rabbit antibody. Nuclei were visualised using DAPI stain. ab3882 gives a predominantly nuclear staining pattern.

    This image is courtesy of an Abreview submitted by Kirk McManus.

    See Abreview

  • All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 120 kDa, 18 kDa. We are unsure as to the identity of these extra bands.

  • Image courtesy of Human Protein Atlas
    ab3882 staining BAF53A in human ovary, showing a distinct and strong staining pattern of the nucleus of the ovarian stromal cells. Paraffin-embedded ovary tissue was incubated with ab3882 (1/35) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab3882 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org


This product has been referenced in:
See all 8 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. However in order to help our customers we have extended our Abpromise guarantee. As per our further conversation if you could get in touch again after selecting few products then I will certainly look into your request for further advice. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I have had the opportunity to inquire about ChIP testing for ab3882 and the image on our datasheet in particular. This image has been provided to us by a collaborator who tested this product in ChIP for us. This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link: https://www.abcam.com/index.html?pageconfig=abpromise I have included some ChIP protocols from our site for your review. More ChIP tips can also be found at this link: www.abcam.com/index.html?pageconfig=resource&rid=310

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Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Embryonic Brain (E15))
Embryonic Brain (E15)
Yes - Triton 0.5%
Blocking step
BSA 0.1% + Normal Goat Serum 10% as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 21 2007

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 05 2006


This antibody has been tested briefly in Immunofluorescence in U20S cells (human). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. No staining was observed. However, staining was only attempted once and this is by no means an indication that the antibody doesn't work in IF, it merely did not perform under the conditions above. Please see reviews and the datasheet for details of any further testing.

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