Overview

  • Product name

    Anti-BAF53b antibody [EP10101]
    See all BAF53b primary antibodies
  • Description

    Rabbit monoclonal [EP10101] to BAF53b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BAF53b aa 350 to the C-terminus.
    Database link: O94805

  • Positive control

    • Human fetal brain lysate; Human and Mouse brain tissues; U87-MG cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab180927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/500.
ICC/IF 1/50.

Target

  • Function

    Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 53 kDa BRG1 associated factor B antibody
    • 53 kDa BRG1-associated factor B antibody
    • ACL 6B antibody
    • ACL6B antibody
    • ACL6B_HUMAN antibody
    • Actin like 6 antibody
    • Actin like protein 6B antibody
    • Actin like protein6B antibody
    • Actin related protein antibody
    • Actin related protein Baf53b antibody
    • actin-like 6B antibody
    • Actin-like protein 6B antibody
    • Actin-related protein Baf53b antibody
    • Actl 6 antibody
    • ACTL 6B antibody
    • Actl6 antibody
    • ACTL6 B antibody
    • Actl6b antibody
    • Arp Na antibody
    • ArpN a antibody
    • ArpN alpha antibody
    • ArpNa antibody
    • ArpNalpha antibody
    • Baf 53b antibody
    • BAF53B antibody
    • BRG1-associated factor 53B antibody
    see all

Images

  • Anti-BAF53b antibody [EP10101] (ab180927) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue, labeling BAF53b with ab180927 at 1/500 dilution and counter-stained with Hematoxylin. 

  • Immunohistochemical analysis of paraffin -embedded Human brain tissue, labeling BAF53b with ab180927 at 1/500 dilution and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of U87-MG cells (paraformaldehyde-fixed, 4%) labeling BAF53b with ab180927 at 1//50 dillution followed by Goat anti Rabbit IgG secondary antibody at 1/200 dilution and counter-stained with Dapi (blue).

References

This product has been referenced in:

  • Lee SW  et al. MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts. Dev Cell 46:73-84.e7 (2018). Read more (PubMed: 29974865) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180927.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up