Recombinant
RabMAb

Recombinant Anti-BAF53b antibody [EPR10100(B)] (ab140642)

Overview

  • Product name

    Anti-BAF53b antibody [EPR10100(B)]
    See all BAF53b primary antibodies
  • Description

    Rabbit monoclonal [EPR10100(B)] to BAF53b
  • Host species

    Rabbit
  • Specificity

    ab140642 is likely to cross-react with the homologue ACTL6A protein, due to high homology with the immunogen sequence.
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BAF53b aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, HepG2 and U-87 MG cell lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.
    • Sequence similarities

      Belongs to the actin family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 53 kDa BRG1 associated factor B antibody
      • 53 kDa BRG1-associated factor B antibody
      • ACL 6B antibody
      • ACL6B antibody
      • ACL6B_HUMAN antibody
      • Actin like 6 antibody
      • Actin like protein 6B antibody
      • Actin like protein6B antibody
      • Actin related protein antibody
      • Actin related protein Baf53b antibody
      • actin-like 6B antibody
      • Actin-like protein 6B antibody
      • Actin-related protein Baf53b antibody
      • Actl 6 antibody
      • ACTL 6B antibody
      • Actl6 antibody
      • ACTL6 B antibody
      • Actl6b antibody
      • Arp Na antibody
      • ArpN a antibody
      • ArpN alpha antibody
      • ArpNa antibody
      • ArpNalpha antibody
      • Baf 53b antibody
      • BAF53B antibody
      • BRG1-associated factor 53B antibody
      see all

    Images

    • All lanes : Anti-BAF53b antibody [EPR10100(B)] (ab140642) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Immunofluorescent analysis of HepG2 cells labelling BAF53b with ab140642 at 1/250 dilution.

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T, MCF7, K562)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    20 µg
    Specification
    293T, MCF7, K562
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 10 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hep3B)
    Permeabilization
    Yes - Tween-20
    Specification
    Hep3B
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 10 2016

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Breast cancer tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
    Permeabilization
    Yes - Tween-20
    Specification
    Breast cancer tissue
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 09 2016

    Answer

    It is likely that ab140642 will cross react with ACTL6A.

    Read More

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