Product nameAnti-BAF53b antibody [EPR10100(B)]
See all BAF53b primary antibodies
DescriptionRabbit monoclonal [EPR10100(B)] to BAF53b
Specificityab140642 is likely to cross-react with the homologue ACTL6A protein, due to high homology with the immunogen sequence.
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human BAF53b aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa, K562, HepG2 and U-87 MG cell lysates; HepG2 cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab140642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 47 kDa.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBelongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.
Sequence similaritiesBelongs to the actin family.
- Information by UniProt
- 53 kDa BRG1 associated factor B antibody
- 53 kDa BRG1-associated factor B antibody
- ACL 6B antibody
All lanes : Anti-BAF53b antibody [EPR10100(B)] (ab140642) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : U-87 MG cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Immunofluorescent analysis of HepG2 cells labelling BAF53b with ab140642 at 1/250 dilution.
This product has been referenced in:
- Tibshirani M et al. Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis. Hum Mol Genet 26:4142-4152 (2017). Read more (PubMed: 28973294) »
- Lancaster MA et al. Cerebral organoids model human brain development and microcephaly. Nature 501:373-9 (2013). IHC . Read more (PubMed: 23995685) »