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    baf57smarce1-antibody-ab115512.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HMG Box
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Anti-BAF57/SMARCE1 antibody (ab115512)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (ab115512)
  • Western blot - Anti-BAF57/SMARCE1 antibody (ab115512)
  • Western blot - Anti-BAF57/SMARCE1 antibody (ab115512)

Key features and details

  • Goat polyclonal to BAF57/SMARCE1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-BAF57/SMARCE1 antibody
    See all BAF57/SMARCE1 primary antibodies
  • Description

    Goat polyclonal to BAF57/SMARCE1
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    PPTDPIPEDEKK

    with a Cysteine residue linker, corresponding to C terminal amino acids 399-410 of Human BAF57/SMARCE1 (NP_003070.3).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human prostate tissue; Jurkat lysate; HEK293 lysate overexpressing BAF57/SMARCE1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99.48% Tris buffered saline
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Dog
Application Abreviews Notes
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Domain

    The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q969G3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6605 Human
    • Entrez Gene: 57376 Mouse
    • Entrez Gene: 303518 Rat
    • Omim: 603111 Human
    • SwissProt: Q969G3 Human
    • SwissProt: O54941 Mouse
    • SwissProt: Q56A18 Rat
    • Unigene: 643780 Human
    • Alternative names

      • BAF57 antibody
      • BRG1 associated factor 57 antibody
      • BRG1-associated factor 57 antibody
      • Chromatin remodeling complex BRG1 associated factor 57 antibody
      • FLJ35648 antibody
      • SMARCE 1 antibody
      • SMARCE1 antibody
      • SMCE1_HUMAN antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (ab115512)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (ab115512)
      ab115512 at 3.75ug/ml staining BAF57/SMARCE1 in Human prostate tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
    • Western blot - Anti-BAF57/SMARCE1 antibody (ab115512)
      Western blot - Anti-BAF57/SMARCE1 antibody (ab115512)
      Anti-BAF57/SMARCE1 antibody (ab115512) at 0.2 µg/ml + Jurkat lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 47 kDa



      Primary incubation: 1 hour
    • Western blot - Anti-BAF57/SMARCE1 antibody (ab115512)
      Western blot - Anti-BAF57/SMARCE1 antibody (ab115512)
      All lanes : Anti-BAF57/SMARCE1 antibody (ab115512) at 0.6 µg/ml

      Lane 1 : HEK293 lysate, mock transfection control
      Lane 2 : HEK293 lysate, overexpressing BAF57/SMARCE1

      Predicted band size: 47 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab115512? Please let us know so that we can cite the reference in this datasheet.

    ab115512 has not yet been referenced specifically in any publications.

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