Anti-BAF57/SMARCE1 antibody (ab115512)
Key features and details
- Goat polyclonal to BAF57/SMARCE1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-BAF57/SMARCE1 antibody
See all BAF57/SMARCE1 primary antibodies -
Description
Goat polyclonal to BAF57/SMARCE1 -
Host species
Goat -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide:
PPTDPIPEDEKK
with a Cysteine residue linker, corresponding to C terminal amino acids 399-410 of Human BAF57/SMARCE1 (NP_003070.3). -
Positive control
- Human prostate tissue; Jurkat lysate; HEK293 lysate overexpressing BAF57/SMARCE1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99.48% Tris buffered saline -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
|
WB |
Human
|
All applications |
Mouse
Rat
Dog
|
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 47 kDa.
|
|
IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 47 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Domain
The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6605 Human
- Entrez Gene: 57376 Mouse
- Entrez Gene: 303518 Rat
- Omim: 603111 Human
- SwissProt: Q969G3 Human
- SwissProt: O54941 Mouse
- SwissProt: Q56A18 Rat
- Unigene: 643780 Human
-
Alternative names
- BAF57 antibody
- BRG1 associated factor 57 antibody
- BRG1-associated factor 57 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (ab115512)ab115512 at 3.75ug/ml staining BAF57/SMARCE1 in Human prostate tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
Anti-BAF57/SMARCE1 antibody (ab115512) at 0.2 µg/ml + Jurkat lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Primary incubation: 1 hour -
All lanes : Anti-BAF57/SMARCE1 antibody (ab115512) at 0.6 µg/ml
Lane 1 : HEK293 lysate, mock transfection control
Lane 2 : HEK293 lysate, overexpressing BAF57/SMARCE1
Predicted band size: 47 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab115512 has not yet been referenced specifically in any publications.