Overview

  • Product name

    Anti-BAF57/SMARCE1 antibody
    See all BAF57/SMARCE1 primary antibodies
  • Description

    Rabbit polyclonal to BAF57/SMARCE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human BAF57/SMARCE1 aa 1-411.
    Database link: Q969G3

  • Positive control

    • HL60, K562, SHSY-5Y. SW620 and SKOV3 cell line extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab186824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 47 kDa.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Domain

    The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAF57 antibody
    • BRG1 associated factor 57 antibody
    • BRG1-associated factor 57 antibody
    • Chromatin remodeling complex BRG1 associated factor 57 antibody
    • FLJ35648 antibody
    • SMARCE 1 antibody
    • SMARCE1 antibody
    • SMCE1_HUMAN antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186824. Blue DAPI for nuclear staining.
  • All lanes : Anti-BAF57/SMARCE1 antibody (ab186824) at 1/500 dilution

    Lane 1 : HL60 cell line extract
    Lane 2 : K562 cell line extract
    Lane 3 : SHSY-5Y cell line extract
    Lane 4 : SW620 cell line extract
    Lane 5 : SKOV3 cell line extract

    Predicted band size: 47 kDa

References

ab186824 has not yet been referenced specifically in any publications.

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