Key features and details
- Rabbit polyclonal to BAF57/SMARCE1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BAF57/SMARCE1 antibody
See all BAF57/SMARCE1 primary antibodies
DescriptionRabbit polyclonal to BAF57/SMARCE1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
Recombinant fragment within Human BAF57/SMARCE1 (internal sequence). The exact sequence is proprietary.
Database link: Q969G3
- WB: NIH/3T3, IMR32 and Jurkat whole cell lysates. IHC-P: NCI-H1299 xenograft tissue. ICC/IF: MCF7 and HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
DomainThe HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
- Information by UniProt
- BAF57 antibody
- BRG1 associated factor 57 antibody
- BRG1-associated factor 57 antibody
Anti-BAF57/SMARCE1 antibody (ab228750) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 47 kDa
7.5% SDS-PAGE gel.
All lanes : Anti-BAF57/SMARCE1 antibody (ab228750) at 1/1000 dilution
Lane 1 : IMR32 whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS-PAGE gel.
Paraffin-embedded NCI-H1299 (human) xenograft tissue stained for BAF57/SMARCE1 with ab228750 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for BAF57/SMARCE1 (green) using ab228750 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BAF57/SMARCE1 (green) using ab228750 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Alpha Tubulin, a cytoskeleton marker, is detected with an alpha Tubulin antibody at 1/500 dilution (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228750 has not yet been referenced specifically in any publications.