Overview

  • Product name

    Anti-BAF57/SMARCE1 antibody [EPR8849]
    See all BAF57/SMARCE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8849] to BAF57/SMARCE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BAF57/SMARCE1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • MCF7, HeLa, Jurkat and Raji cell lysates; Human brain tissue; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Domain

    The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAF57 antibody
    • BRG1 associated factor 57 antibody
    • BRG1-associated factor 57 antibody
    • Chromatin remodeling complex BRG1 associated factor 57 antibody
    • FLJ35648 antibody
    • SMARCE 1 antibody
    • SMARCE1 antibody
    • SMCE1_HUMAN antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
    see all

Images

  • All lanes : Anti-BAF57/SMARCE1 antibody [EPR8849] (ab137081) at 1/1000 dilution

    Lane 1 : MCF7 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : Raji cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.
  • Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab137081 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Immunofluorescent analysis of MCF7 cells labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.

References

This product has been referenced in:

  • Cabot B  et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). ICC/IF . Read more (PubMed: 29024220) »
See 1 Publication for this product

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