Key features and details
- Rabbit polyclonal to BAF60C
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BAF60C antibody
See all BAF60C primary antibodies
DescriptionRabbit polyclonal to BAF60C
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: HEK-293 whole cell lysates; IHC-P: Human heart muscle tissue; ICC: SH-SY5Y whole cells.
Previously labelled as SMARCD3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab204745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionPlays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityIsoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
Sequence similaritiesBelongs to the SMARCD family.
Contains 1 SWIB domain.
- Information by UniProt
- 60 kDa BRG 1/Brm associated factor subunit C antibody
- 60 kDa BRG-1/Brm-associated factor subunit C antibody
- BAF60C antibody
All lanes : Anti-BAF60C antibody (ab204745) at 0.4 µg/ml
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Predicted band size: 55 kDa
Corresponding SMARCD3 RNA-seq data specify in the bottom of image.
Loading control: Anti-GAPDH.
Immunocytochemical analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cells labeling BAF60C in the nucleoplasm (green) with ab204745 at 2 µg/ml.
Immunohistochemical analysis of paraffin-embedded human heart muscle tissue labeling BAF60C in the nucleus of myocytes with ab204745 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab204745 has not yet been referenced specifically in any publications.