Product nameAnti-BAF60C antibody [EPR5538(2)]
See all BAF60C primary antibodies
DescriptionRabbit monoclonal [EPR5538(2)] to BAF60C
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
within Human BAF60C aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q6STE5
- Purchase matching WB positive control:Recombinant Human BAF60C protein
- HeLa, JAR, C6 and NIH/3T3 cell lysates, paraffin-embedded endometrial adenocarcinoma tissue, paraffin-embedded trachea tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab171075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionPlays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Tissue specificityIsoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
Sequence similaritiesBelongs to the SMARCD family.
Contains 1 SWIB domain.
- Information by UniProt
- 60 kDa BRG 1/Brm associated factor subunit C antibody
- 60 kDa BRG-1/Brm-associated factor subunit C antibody
- BAF60C antibody
All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
Immunohistochemical analysis of paraffin-embedded endometrial adenocarcinoma tissue labeling BAF60C with ab171075 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded trachea tissue labeling BAF60C with ab171075 at 1/10 dilution.
Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/10 dilution
Lane 1 : Immunoprecipitation pellet from HeLa cell lysate
Lane 2 : 1x PBS
All lanes : HRP-conjugated anti-rabbit IgG
ab171075 has not yet been referenced specifically in any publications.