• Product name

    Anti-BAF60C antibody [EPR5538(2)]
    See all BAF60C primary antibodies
  • Description

    Rabbit monoclonal [EPR5538(2)] to BAF60C
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human BAF60C aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6STE5

  • Positive control

  • General notes

    Previously labelled as SMARCD3.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


Our Abpromise guarantee covers the use of ab171075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Plays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • Tissue specificity

      Isoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
    • Sequence similarities

      Belongs to the SMARCD family.
      Contains 1 SWIB domain.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • 60 kDa BRG 1/Brm associated factor subunit C antibody
      • 60 kDa BRG-1/Brm-associated factor subunit C antibody
      • BAF60C antibody
      • BRG1 associated factor 60C antibody
      • BRG1-associated factor 60C antibody
      • Chromatin remodeling complex BAF60C subunit antibody
      • CRACD3 antibody
      • Mammalian chromatin remodeling complex BRG1 associated factor 60C antibody
      • MGC111010 antibody
      • Rsc6p antibody
      • Smarcd3 antibody
      • SMRD3_HUMAN antibody
      • SWI/SNF complex 60 kDa subunit C antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 3 antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3, isoform 1 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 antibody
      • Swp73 like protein antibody
      see all


    • All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa

    • Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue labeling BAF60C with ab171075 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded human trachea tissue labeling BAF60C with ab171075 at 1/10 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/10 dilution

      Lane 1 : Immunoprecipitation pellet from HeLa cell lysate
      Lane 2 : 1x PBS

      All lanes : HRP-conjugated anti-rabbit IgG


    This product has been referenced in:

    • Nakamura R  et al. Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice. Dev Growth Differ 58:367-82 (2016). Read more (PubMed: 27125315) »
    See 1 Publication for this product

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