- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-BAFF antibody
See all BAFF primary antibodies -
DescriptionRabbit polyclonal to BAFF
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to amino acids 254-269 of Human BAFF.
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Positive control
- Purchase matching WB positive control:Recombinant Human BAFF protein
- HL60 cell lysate
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General notesBAFF is also known as B cell Activating Factor
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityIon Exchange Chromatography
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ClonalityPolyclonal
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IsotypeIgG
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab8396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with BAFF peptide (ab8397). |
Target
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FunctionCytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
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Tissue specificityAbundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 10673 Human
- Entrez Gene: 24099 Mouse
- Entrez Gene: 498666 Rat
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- SwissProt: Q9WU72 Mouse
- Unigene: 525157 Human
- Unigene: 596110 Human
see all -
Alternative names
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
Images
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Western blot - Anti-BAFF antibody (ab8396)All lanes : Anti-BAFF antibody (ab8396) at 1 µg/ml
Lane 1 : Human HL60 cell lysate
Lane 2 : Murine spleen tissue lysate
Observed band size: 34 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody (ab8396)ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF
References
This product has been referenced in:
- Walls A et al. Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus. Int J Pediatr Otorhinolaryngol 80:49-52 (2016). WB ; Human . Read more (PubMed: 26746612) »
- Panchanathan R & Choubey D Murine BAFF expression is up-regulated by estrogen and interferons: implications for sex bias in the development of autoimmunity. Mol Immunol 53:15-23 (2013). Read more (PubMed: 22784990) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"