Anti-BAFF antibody (ab8396)

Submit a review Q&A (3)References (3)
Western blot - Anti-BAFF antibody (ab8396)
  • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody (ab8396)

Key features and details

  • Rabbit polyclonal to BAFF
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-BAFF antibody [EPR22238] (ab224710)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-BAFF antibody
    See all BAFF primary antibodies

  • Description

    Rabbit polyclonal to BAFF

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to amino acids 254-269 of Human BAFF.

  • Positive control

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.

Target

  • Function

    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

  • Tissue specificity

    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ApoL related ligand TALL 1 antibody
    • B cell Activating Factor antibody
    • B lymphocyte stimulator antibody
    • B-cell-activating factor antibody
    • BAFF antibody
    • BLyS antibody
    • CD 257 antibody
    • CD257 antibody
    • CD257 antigen antibody
    • Delta BAFF antibody
    • Dendritic cell derived TNF like molecule antibody
    • Dendritic cell-derived TNF-like molecule antibody
    • DTL antibody
    • DTL precursor antibody
    • PRO738 antibody
    • soluble form antibody
    • TALL 1 antibody
    • TALL-1 antibody
    • TALL1 antibody
    • THANK antibody
    • TN13B_HUMAN antibody
    • TNF and APOL related leukocyte expressed ligand 1 antibody
    • TNF homolog that activates apoptosis antibody
    • TNF homolog that activates apoptosis NKFB and JNK. antibody
    • TNF- and APOL-related leukocyte expressed ligand 1 antibody
    • TNFSF13B antibody
    • TNFSF20 antibody
    • TNLG7A antibody
    • Tumor necrosis factor (ligand) superfamily member 13b antibody
    • Tumor necrosis factor ligand 7A antibody
    • Tumor necrosis factor ligand superfamily member 13b antibody
    • Tumor necrosis factor ligand superfamily member 20 antibody
    • Tumor necrosis factor like protein ZTNF4 antibody
    • Tumor necrosis factor superfamily member 13B antibody
    • UNQ401 antibody
    • ZTNF4 antibody
    see all

Images

  • Western blot - Anti-BAFF antibody (ab8396)
    Western blot - Anti-BAFF antibody (ab8396)
    All lanes : Anti-BAFF antibody (ab8396) at 1 µg/ml

    Lane 1 : Human HL60 cell lysate
    Lane 2 : Murine spleen tissue lysate

    Observed band size: 34 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody (ab8396)
    Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody (ab8396)

    ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF

References (3)

Publishing research using ab8396? Please let us know so that we can cite the reference in this datasheet.

ab8396 has been referenced in 3 publications.

  • Cao Y  et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
  • Walls A  et al. Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus. Int J Pediatr Otorhinolaryngol 80:49-52 (2016). WB ; Human . PubMed: 26746612
  • Panchanathan R & Choubey D Murine BAFF expression is up-regulated by estrogen and interferons: implications for sex bias in the development of autoimmunity. Mol Immunol 53:15-23 (2013). PubMed: 22784990

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Thank you for caring and providing an excellent service (as always).
The customer has tried to implement Abcam's suggestion but still got unspecific results.
We would very much appreciate to go ahead with your offer to get a replacement antibody in this case.
If a replacement is possible, please wait for my input regarding required antibody.

Thanks again for your kind help.
Regards,

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Answer

Thank you for your reply.

I will be happy to send a replacement if the product is still under the guarantee Abpromise (after 6 months of the purchase date). Could you please provide the order number?

Would you prefer receiving a new lot from the same product, or a different product against the same target?

Please let me know if you require any further assistance.

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Question

Dear Tech support,   One of our customers has ordered ab8396 and needs a positive control for it for WB. Which of the available Baff protein/peptides would you recommend? The product ab8397 seems to have the exact amino acid sequence used as an immonogen for ab8396 yet is only stated to have been tested for blocking, would this product be a good control and can it be guaranteed by abpromise for that application.   Thank you for your kind help. Regards,  

Read More
Answer

Thank you for your inquiry. The ab8396 can only be used for blocking. Indeed, the peptide is very short containing only 16 amino acids. I would therefore not recommend it for using as a control by loading on the gel. It would run at around 1.8kD - which is very low and which needs a special gel. As a positive control for BAFF and for ab8396 I can recommend either lysates - such as spleen lysate (e.g. ab29699 : human / ab7937:mouse) or a full length protein containing the immunogen region of ab8396. We do have several full length proteins which we all do guarantee for this application with our Abpromise guarantee:  ab79745,  ab83938, ab109136.  Click here (or use the following: https://www.abcam.com/Recombinant-human-BAFF-protein-ab79745.html). Click here (or use the following: https://www.abcam.com/BAFF-protein-Active-ab83938.html). Click here (or use the following: https://www.abcam.com/Recombinant-human-BAFF-protein-ab109136.html). I hope this is helpful. Please do not hesitate to get back to us if you need some more information.

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Question

I need to find a Baff antibody which can be used for IHC. Has ab7357 or ab8396 been tested for IHC? Thanks.

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Answer

Thank you for your enquiry. To our knowledge, neither of these antibodies have yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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