• Product name
    Anti-BAFF antibody [Buffy 2]
    See all BAFF primary antibodies
  • Description
    Rat monoclonal [Buffy 2] to BAFF
  • Host species
  • Specificity
    Recognizes membrane-bound and soluble human and mouse (although weaker in mouse) BAFF.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant soluble protein, corresponding to amino acids 83 - 285 of Human BAFF. It recognizes the TNF-homology domain.

  • Positive control



Our Abpromise guarantee covers the use of ab16081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration.


  • Function
    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
  • Tissue specificity
    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ApoL related ligand TALL 1 antibody
    • B cell Activating Factor antibody
    • B lymphocyte stimulator antibody
    • B-cell-activating factor antibody
    • BAFF antibody
    • BLyS antibody
    • CD 257 antibody
    • CD257 antibody
    • CD257 antigen antibody
    • Delta BAFF antibody
    • Dendritic cell derived TNF like molecule antibody
    • Dendritic cell-derived TNF-like molecule antibody
    • DTL antibody
    • DTL precursor antibody
    • PRO738 antibody
    • soluble form antibody
    • TALL 1 antibody
    • TALL-1 antibody
    • TALL1 antibody
    • THANK antibody
    • TN13B_HUMAN antibody
    • TNF and APOL related leukocyte expressed ligand 1 antibody
    • TNF homolog that activates apoptosis antibody
    • TNF homolog that activates apoptosis NKFB and JNK. antibody
    • TNF- and APOL-related leukocyte expressed ligand 1 antibody
    • TNFSF13B antibody
    • TNFSF20 antibody
    • TNLG7A antibody
    • Tumor necrosis factor (ligand) superfamily member 13b antibody
    • Tumor necrosis factor ligand 7A antibody
    • Tumor necrosis factor ligand superfamily member 13b antibody
    • Tumor necrosis factor ligand superfamily member 20 antibody
    • Tumor necrosis factor like protein ZTNF4 antibody
    • Tumor necrosis factor superfamily member 13B antibody
    • UNQ401 antibody
    • ZTNF4 antibody
    see all


  • Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using ab16081.

    Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

  • ICC/IF image of ab16081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16081, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing THP1 cells stained with ab16081 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16081, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgM (mu chain) (ab98368) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgM (ab35768, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.


This product has been referenced in:
  • Gerzanich V  et al. Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis. J Neuroinflammation 14:177 (2017). Read more (PubMed: 28865458) »
  • Müller N  et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . Read more (PubMed: 24728308) »

See all 9 Publications for this product

Customer reviews and Q&As

We have many products in catalogue. Please find below few of these




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According to our records, ab16081 and ab16232 was proving difficult to use when staining spleen and aorta tissues presumably in immunohistochemistry and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appea...

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Le laboratoire m'a confirmé que de récents tests ont montré que l'anticorps fonctionne en effet en cytométrie en flux pour le marquage extracellulaire et intracellulaire également.

La fiche technique de l'ant...

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Merci de nous avoir contactés.

Il a été décrit que le clone [Buffy 2] ne distingue pas les 2 formes de la BAFF et peut donc reconnaître à la fois la forme soluble et la forme fixée à la me...

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I am sorry to hear that you have experienced difficulties with two of our products in the past. However, because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our custo...

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Thank you for your reply.

I am sorry that the vials you received did not contain the full amount of the product and I apologize for the inconvenience. As requested, I have issued a free of charge replacement vials of ab16081 and ab16232 with...

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Thank you for contacting us.

I have some further information to share with you in regards to the antibodies ab119904, ab79056 and ab86574.

Anti-CD134 / OX40 (ab119904)

This antibody was used in staining of paraffin embedde...

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Thank you for contacting us.

Your credit note ID is 20547.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please a...

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Thank you for your reply.

Regarding the three options mentioned, product ab65360 is not currently in stock, and it is yet to be confirmed the estimated time of arrival.

Product ab16081 is a good seller with low rate of complaints, b...

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Thank you for your patience with this reply. I finally heard back from the lab as they were looking into this further. The production manager said that IgMs really are pentameric and nothing else, but it is possible that there could potentially...

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