Recombinant
RabMAb

Recombinant Anti-BAFF antibody [EPR19704] (ab203791)

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Overview

  • Product name

    Anti-BAFF antibody [EPR19704]
    See all BAFF primary antibodies

  • Description

    Rabbit monoclonal [EPR19704] to BAFF

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human BAFF aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y275

  • Positive control

    • WB: HEK-293 transfected with a GFP-Myc tagged BAFF vector whole cell lysate; U937 whole cell lysate. ICC/IF: 293T cells transfected with a GFP-tagged BAFF expression construct. IP: U937 whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/30.
WB 1/1000. Detects a band of approximately 31, 29, 17 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

  • Tissue specificity

    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ApoL related ligand TALL 1 antibody
      • B cell Activating Factor antibody
      • B lymphocyte stimulator antibody
      • B-cell-activating factor antibody
      • BAFF antibody
      • BLyS antibody
      • CD 257 antibody
      • CD257 antibody
      • CD257 antigen antibody
      • Delta BAFF antibody
      • Dendritic cell derived TNF like molecule antibody
      • Dendritic cell-derived TNF-like molecule antibody
      • DTL antibody
      • DTL precursor antibody
      • PRO738 antibody
      • soluble form antibody
      • TALL 1 antibody
      • TALL-1 antibody
      • TALL1 antibody
      • THANK antibody
      • TN13B_HUMAN antibody
      • TNF and APOL related leukocyte expressed ligand 1 antibody
      • TNF homolog that activates apoptosis antibody
      • TNF homolog that activates apoptosis NKFB and JNK. antibody
      • TNF- and APOL-related leukocyte expressed ligand 1 antibody
      • TNFSF13B antibody
      • TNFSF20 antibody
      • TNLG7A antibody
      • Tumor necrosis factor (ligand) superfamily member 13b antibody
      • Tumor necrosis factor ligand 7A antibody
      • Tumor necrosis factor ligand superfamily member 13b antibody
      • Tumor necrosis factor ligand superfamily member 20 antibody
      • Tumor necrosis factor like protein ZTNF4 antibody
      • Tumor necrosis factor superfamily member 13B antibody
      • UNQ401 antibody
      • ZTNF4 antibody
      see all

    Images

    • Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      All lanes : Anti-BAFF antibody [EPR19704] (ab203791) at 1/5000 dilution

      Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with a GFP-Myc tagged BAFF vector whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 57 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

       

    • Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      Anti-BAFF antibody [EPR19704] (ab203791) at 1/1000 dilution + U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 17,29,31 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Two isoforms have been described in literature (PMID: 12867412).

    • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (ab203791)
      Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (ab203791)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells labeling BAFF with ab203791 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.

      Confocal image showing positive staining on 293T cells transfected with a GFP-tagged BAFF expression construct.

      The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 at 1/1000 dilution.

      ICC/IF was also performed on a few cell lines including U937, no signal was detected.

    • Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)
      Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)

      BAFF was immunoprecipitated from 0.35 mg of U937 (Human histiocytic lymphoma cell line) whole cell lysate with ab203791 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203791 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: U937 whole cell lysate, 10µg (Input).

      Lane 2: ab203791 IP in U937 whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab203791 in U937 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    References

    ab203791 has not yet been referenced specifically in any publications.

    Publishing research using ab203791? Please let us know so that we can cite the reference in this datasheet.

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