Recombinant Anti-BAFF antibody [EPR19704] (ab203791)
![Western blot - Anti-BAFF antibody [EPR19704] (ab203791)](https://www.abcam.com/ps/products/203/ab203791/Images/ab203791-266359-anti-baff-antibody-epr19704-western-blot.jpg "Western blot - Anti-BAFF antibody [EPR19704] (ab203791)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19704] to BAFF
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-BAFF antibody [EPR19704]
See all BAFF primary antibodies -
Description
Rabbit monoclonal [EPR19704] to BAFF -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293 transfected with a GFP-Myc tagged BAFF vector whole cell lysate; U937 whole cell lysate. ICC/IF: 293T cells transfected with a GFP-tagged BAFF expression construct. IP: U937 whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19704 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
|
| IP |
1/30.
|
|
| WB |
1/1000. Detects a band of approximately 31, 29, 17 kDa (predicted molecular weight: 31 kDa).
|
| Notes |
|---|
|
ICC/IF
1/100. |
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 31, 29, 17 kDa (predicted molecular weight: 31 kDa). |
Target
-
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
-
Alternative names
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
Images
-
Western blot - Anti-BAFF antibody [EPR19704] (ab203791)All lanes : Anti-BAFF antibody [EPR19704] (ab203791) at 1/5000 dilution
Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with a GFP-Myc tagged BAFF vector whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-BAFF antibody [EPR19704] (ab203791)](https://www.abcam.com/ps/products/203/ab203791/Images/ab203791-266358-anti-baff-antibody-epr19704-western-blot.jpg)
Western blot - Anti-BAFF antibody [EPR19704] (ab203791)Anti-BAFF antibody [EPR19704] (ab203791) at 1/1000 dilution + U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 17,29,31 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Two isoforms have been described in literature (PMID: 12867412).
-
![Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (ab203791)](https://www.abcam.com/ps/products/203/ab203791/Images/ab203791-266357-anti-baff-antibody-epr19704-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (ab203791)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells labeling BAFF with ab203791 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.
Confocal image showing positive staining on 293T cells transfected with a GFP-tagged BAFF expression construct.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 at 1/1000 dilution.
ICC/IF was also performed on a few cell lines including U937, no signal was detected.
-
![Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)](https://www.abcam.com/ps/products/203/ab203791/Images/ab203791-266356-anti-baff-antibody-epr19704-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)BAFF was immunoprecipitated from 0.35 mg of U937 (Human histiocytic lymphoma cell line) whole cell lysate with ab203791 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203791 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: U937 whole cell lysate, 10µg (Input).
Lane 2: ab203791 IP in U937 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab203791 in U937 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
-
![Anti-BAFF antibody [EPR19704] (ab203791)](https://www.abcam.com/ps/products/203/ab203791/Images/ab203791-6-benefits-of-recombinant-antibodies.png)
Anti-BAFF antibody [EPR19704] (ab203791)
Protocols
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab203791 has not yet been referenced specifically in any publications.
