RecombinantRabMAb

Recombinant Anti-BAFF antibody [EPR19704] (ab203791)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19704] to BAFF
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BAFF antibody [EPR19704]
    See all BAFF primary antibodies

  • Description

    Rabbit monoclonal [EPR19704] to BAFF

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human BAFF aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y275

  • Positive control

    • WB: HEK-293 transfected with a GFP-Myc tagged BAFF vector whole cell lysate; U937 whole cell lysate. ICC/IF: 293T cells transfected with a GFP-tagged BAFF expression construct. IP: U937 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab203791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/30.
WB 1/1000. Detects a band of approximately 31, 29, 17 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

  • Tissue specificity

    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ApoL related ligand TALL 1 antibody
      • B cell Activating Factor antibody
      • B lymphocyte stimulator antibody
      • B-cell-activating factor antibody
      • BAFF antibody
      • BLyS antibody
      • CD 257 antibody
      • CD257 antibody
      • CD257 antigen antibody
      • Delta BAFF antibody
      • Dendritic cell derived TNF like molecule antibody
      • Dendritic cell-derived TNF-like molecule antibody
      • DTL antibody
      • DTL precursor antibody
      • PRO738 antibody
      • soluble form antibody
      • TALL 1 antibody
      • TALL-1 antibody
      • TALL1 antibody
      • THANK antibody
      • TN13B_HUMAN antibody
      • TNF and APOL related leukocyte expressed ligand 1 antibody
      • TNF homolog that activates apoptosis antibody
      • TNF homolog that activates apoptosis NKFB and JNK. antibody
      • TNF- and APOL-related leukocyte expressed ligand 1 antibody
      • TNFSF13B antibody
      • TNFSF20 antibody
      • TNLG7A antibody
      • Tumor necrosis factor (ligand) superfamily member 13b antibody
      • Tumor necrosis factor ligand 7A antibody
      • Tumor necrosis factor ligand superfamily member 13b antibody
      • Tumor necrosis factor ligand superfamily member 20 antibody
      • Tumor necrosis factor like protein ZTNF4 antibody
      • Tumor necrosis factor superfamily member 13B antibody
      • UNQ401 antibody
      • ZTNF4 antibody
      see all

    Images

    • Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      All lanes : Anti-BAFF antibody [EPR19704] (ab203791) at 1/5000 dilution

      Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with a GFP-Myc tagged BAFF vector whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 57 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

       

    • Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      Western blot - Anti-BAFF antibody [EPR19704] (ab203791)
      Anti-BAFF antibody [EPR19704] (ab203791) at 1/1000 dilution + U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 17,29,31 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Two isoforms have been described in literature (PMID: 12867412).

    • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (ab203791)
      Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (ab203791)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells labeling BAFF with ab203791 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.

      Confocal image showing positive staining on 293T cells transfected with a GFP-tagged BAFF expression construct.

      The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 at 1/1000 dilution.

      ICC/IF was also performed on a few cell lines including U937, no signal was detected.

    • Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)
      Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)

      BAFF was immunoprecipitated from 0.35 mg of U937 (Human histiocytic lymphoma cell line) whole cell lysate with ab203791 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203791 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: U937 whole cell lysate, 10µg (Input).

      Lane 2: ab203791 IP in U937 whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab203791 in U937 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Anti-BAFF antibody [EPR19704] (ab203791)
      Anti-BAFF antibody [EPR19704] (ab203791)

    References (0)

    Publishing research using ab203791? Please let us know so that we can cite the reference in this datasheet.

    ab203791 has not yet been referenced specifically in any publications.

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