Recombinant
RabMAb

Recombinant Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)

Overview

  • Product name

    Anti-BAFF antibody [EPR22238] - BSA and Azide free
    See all BAFF primary antibodies
  • Description

    Rabbit monoclonal [EPR22238] to BAFF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human BAFF aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y275

  • Positive control

    • ICC/IF: U937 cells. Flow Cyt: HL-60 cells. IP: U937 whole cell lysate.
  • General notes

    Ab245833 is the carrier-free version of ab224710. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245833 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30, 32 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
    • Tissue specificity

      Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
      N-glycosylated.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ApoL related ligand TALL 1 antibody
      • B cell Activating Factor antibody
      • B lymphocyte stimulator antibody
      • B-cell-activating factor antibody
      • BAFF antibody
      • BLyS antibody
      • CD 257 antibody
      • CD257 antibody
      • CD257 antigen antibody
      • Delta BAFF antibody
      • Dendritic cell derived TNF like molecule antibody
      • Dendritic cell-derived TNF-like molecule antibody
      • DTL antibody
      • DTL precursor antibody
      • PRO738 antibody
      • soluble form antibody
      • TALL 1 antibody
      • TALL-1 antibody
      • TALL1 antibody
      • THANK antibody
      • TN13B_HUMAN antibody
      • TNF and APOL related leukocyte expressed ligand 1 antibody
      • TNF homolog that activates apoptosis antibody
      • TNF homolog that activates apoptosis NKFB and JNK. antibody
      • TNF- and APOL-related leukocyte expressed ligand 1 antibody
      • TNFSF13B antibody
      • TNFSF20 antibody
      • TNLG7A antibody
      • Tumor necrosis factor (ligand) superfamily member 13b antibody
      • Tumor necrosis factor ligand 7A antibody
      • Tumor necrosis factor ligand superfamily member 13b antibody
      • Tumor necrosis factor ligand superfamily member 20 antibody
      • Tumor necrosis factor like protein ZTNF4 antibody
      • Tumor necrosis factor superfamily member 13B antibody
      • UNQ401 antibody
      • ZTNF4 antibody
      see all

    Images

    • BAFF was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab224710 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224710 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: U937 whole cell lysate 10 µg (Input). 

      Lane 2: ab224710 IP in U937 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224710 in U937 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 20 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224710).

    • Flow cytometric analysis of Daudi (human Burkitt's lymphoma cell line; left panel) and HL-60 (human promyelocytic leukemia cell line, right panel) cell lines labeling BAFF with ab224710 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: Daudi. (PMID: 20951740)

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224710).

    • Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling BAFF with ab224710 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Confocal image showing membranous staining in U-937 cells.

      We don’t recommend PFA fixation, since the result shows some background staining in the nuclear.

      Negative control: HEK-293 PMID: 17393395.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224710).

    References

    ab245833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245833.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up