Overview

  • Product name
    Anti-BAFF-R antibody [11C1]
    See all BAFF-R primary antibodies
  • Description
    Mouse monoclonal [11C1] to BAFF-R
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human BAFF-R. transfected mouse B cell lymphoma L1.2 cells

  • Positive control
    • IHC-P: human tonsil FACS: CD19+ B cells from tonsil.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    11C1
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Flow Cyt
IHC-Fr
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. Antigen retrieval with EDTA based solution (pH9.0) and heat.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
    • Tissue specificity
      Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.
    • Involvement in disease
      Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities
      Contains 1 TNFR-Cys repeat.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell activating factor receptor antibody
      • B-cell-activating factor receptor antibody
      • BAFF R antibody
      • BAFF receptor antibody
      • BAFF-R antibody
      • BAFFR antibody
      • BLyS receptor 3 antibody
      • BlySR3 antibody
      • BR 3 antibody
      • BR3 antibody
      • BROMIX antibody
      • CD 268 antibody
      • CD268 antibody
      • CD268 antigen antibody
      • CVID4 antibody
      • MGC138235 antibody
      • OTTHUMP00000028746 antibody
      • Prolixin antibody
      • TNFRSF 13C antibody
      • Tnfrsf13c antibody
      • TR13C_HUMAN antibody
      • Tumor necrosis factor receptor subunit member 13C antibody
      • Tumor necrosis factor receptor superfamily member 13C antibody
      see all

    Images

    • Immunohistochemistry staining of human tonsil with ab16232, showing intense staining of B cell follicles and weaker staining on GC. Magnification X200.
    • FACS analysis of B cells performed by gating on CD19+ B cells from tonsil.

    References

    This product has been referenced in:
    • Chong BF  et al. Differential expression of BAFF and its receptors in discoid lupus erythematosus patients. J Dermatol Sci N/A:N/A (2013). Human . Read more (PubMed: 24315762) »
    • Li YJ  et al. Expression of BAFF and BAFF-R in follicular lymphoma: correlation with clinicopathologic characteristics and survival outcomes. PLoS One 7:e50936 (2012). IHC . Read more (PubMed: 23272079) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    According to our records, ab16081 and ab16232 was proving difficult to use when staining spleen and aorta tissues presumably in immunohistochemistry and we were in contact in order to help resolve the issue.
    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution. I have attached a questionnaire which will help you to explain the problems encountered with these antibodies and will allow us to investigate the case more closely. Alternatively, you could submit your complaint by filling out the enquiry form found at the following web address:
    https://www.abcam.com/index.html?pageconfig=inquiry
    If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.
    We wish you the best of luck with your research and look forward to a reply.

    Read More

    Answer

    I am sorry to hear that you have experienced difficulties with two of our products in the past. However, because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.
    Regarding the problems encountered with ab16081 and ab16232, if you would like to contact scientific support with information about the protocol used and what difficulties you encountered, we would be happy to help.
    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.
    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
    To find out more about our Abreview system, please see the following link:
    https://www.abcam.com/abreviews
    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Question
    Answer

    Thank you for your reply.

    I am sorry that the vials you received did not contain the full amount of the product and I apologize for the inconvenience. As requested, I have issued a free of charge replacement vials of ab16081 and ab16232 with the order number ######.

    Please do not hesitate to contact us if you need anything further.

    Read More

    Question
    Answer

    I'm afraid the the epitope for ab16232 has not been mapped. Sorry I couldn't help you more on this occasion,

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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