Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
Key features and details
- Highly potent, selective (V)-ATPase inhibitor
- CAS Number: 88899-55-2
- Soluble in ethanol to 5mM
- Form / State: Solid
- Source: Streptomyces griseus
Overview
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Product name
Bafilomycin A1, (V)-ATPase inhibitor -
Description
Highly potent, selective (V)-ATPase inhibitor -
Biological description
Highly potent, selective vacuolar (V)-ATPase inhibitor (IC50 = 500 pM in chromaffin granule membranes). Induces vacuolar deacidification and promotes apoptosis.
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CAS Number
88899-55-2 -
Chemical structure
Properties
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Chemical name
(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one -
Molecular weight
622.84 -
Molecular formula
C35H58O9 -
PubChem identifier
6436223 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 5mM -
Handling
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O[C@@]1(C[C@@H](O)[C@H](C)[C@H](O1)C(C)C)[C@@H](C)[C@H](O)[C@H](C)C2OC(=O)C(OC)=CC=C(C)[C@@H](C)[C@@H](O)[C@@H](C)CC(C)=CC=C[C@@H]2OC -
Source
Streptomyces griseus
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Research areas
Associated products
Images
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Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
ab155686 staining SQSTM1/p62 (autophagosome) in control HeLa cells treated with ethanol 0.1% (left panels) and SQSTM1/p62 in HeLa cells treated with 1uM bafilomycin A1 (ab120497) for 18hrs (right panels). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab155686 at 1/500 dilution and ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)ab109012 staining SQSTM1/p62 (autophagosome) in control HeLa cells treated with ethanol 0.1% (left panels) and SQSTM1/p62 in HeLa cells treated with 1uM bafilomycin A1 (ab120497) for 18hrs (right panels). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab109012 at 5ug/ml and ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (31)
ab120497 has been referenced in 31 publications.
- Wauters F et al. LRRK2 mutations impair depolarization-induced mitophagy through inhibition of mitochondrial accumulation of RAB10. Autophagy 16:203-222 (2020). PubMed: 30945962
- Wu X et al. Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Cell Chem Biol 27:571-585.e6 (2020). PubMed: 32243810
- Xavier A et al. hGBP1 Coordinates Chlamydia Restriction and Inflammasome Activation through Sequential GTP Hydrolysis. Cell Rep 31:107667 (2020). PubMed: 32433976
- Zhen Y et al. Flubendazole elicits anti-cancer effects via targeting EVA1A-modulated autophagy and apoptosis in Triple-negative Breast Cancer. Theranostics 10:8080-8097 (2020). PubMed: 32724459
- Nakai N et al. Autophagy under glucose starvation enhances protein translation initiation in response to re-addition of glucose in C2C12 myotubes. FEBS Open Bio 10:2149-2156 (2020). PubMed: 32882752
