Overview

  • Product name

  • Description

    Rabbit polyclonal to Bag1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Bag1 (internal sequence). The exact sequence is proprietary.
    Database link: Q99933

  • Positive control

    • IHC-P: A549 xenograft tissue. WB: A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.

Target

  • Function

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
  • Tissue specificity

    Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
  • Sequence similarities

    Contains 1 BAG domain.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bag 1 antibody
    • BAG family molecular chaperone regulator 1 antibody
    • BAG-1 antibody
    • BAG1 antibody
    • BAG1_HUMAN antibody
    • BCL 2 Associated Athanogene 1 antibody
    • BCL 2 associated athanogene antibody
    • BCL 2 binding athanogene 1 antibody
    • Bcl-2-associated athanogene 1 antibody
    • BCL2 associated athanogene 1 antibody
    • BCL2 associated athanogene antibody
    • Glucocorticoid receptor-associated protein RAP46 antibody
    • HAP1 antibody
    • Haptoglobin antibody
    • RAP 46 antibody
    • RAP46 antibody
    see all

Images

  • Anti-Bag1 antibody (ab227263) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded A549 xenograft tissue stained for Bag1 using ab227263 at 1/100 dilution in immunohistochemical analysis.

References

ab227263 has not yet been referenced specifically in any publications.

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