Recombinant Anti-Bag1 antibody [Y166] (ab32109)
![Western blot - Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109-43912-anti-bag1-antibody-y166-western-blot.gif "Western blot - Anti-Bag1 antibody [Y166] (ab32109)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y166] to Bag1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Bag1 antibody [Y166]
See all Bag1 primary antibodies -
Description
Rabbit monoclonal [Y166] to Bag1 -
Host species
Rabbit -
Specificity
The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S). -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa cell lysate and cells, human prostate carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y166 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa).
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt.
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. -
Tissue specificity
Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). -
Sequence similarities
Contains 1 BAG domain.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 573 Human
- Entrez Gene: 12017 Mouse
- Entrez Gene: 297994 Rat
- Omim: 601497 Human
- SwissProt: Q99933 Human
- SwissProt: Q60739 Mouse
- SwissProt: B0K019 Rat
- Unigene: 377484 Human
see all -
Alternative names
- Bag 1 antibody
- BAG family molecular chaperone regulator 1 antibody
- BAG-1 antibody
see all
Images
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Western blot - Anti-Bag1 antibody [Y166] (ab32109)Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate
Predicted band size: 31 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa (possible isoform), 50 kDa (possible isoform) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109-43911-anti-bag1-antibody-y166-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109-43910-anti-bag1-antibody-y166-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution. -
![Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109-4-benefits-of-recombinant-antibodies.png)
Anti-Bag1 antibody [Y166] (ab32109)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (8)
ab32109 has been referenced in 8 publications.
- Gennaro VJ et al. Interaction between the BAG1S isoform and HSP70 mediates the stability of anti-apoptotic proteins and the survival of osteosarcoma cells expressing oncogenic MYC. BMC Cancer 19:258 (2019). PubMed: 30902071
- Miao JT et al. LncRNA ANRIL affects the sensitivity of ovarian cancer to cisplatin via regulation of let-7a/HMGA2 axis. Biosci Rep 39:N/A (2019). PubMed: 31189742
- Lu S et al. Downregulation of BAG-1 in T47D cells promotes resistance to tamoxifen via activation of the PI3K/Akt/mTOR signaling pathway. Oncol Rep 41:1901-1910 (2019). PubMed: 30747221
- Shen Z et al. miR-494-BAG-1 axis is involved in cinobufacini-induced cell proliferation and apoptosis in gastric cancer. Mol Med Rep 17:7435-7441 (2018). PubMed: 29568970
- Wood RJ et al. A biosensor-based framework to measure latent proteostasis capacity. Nat Commun 9:287 (2018). PubMed: 29348634
- Cato L et al. Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer. Elife 6:N/A (2017). IHC-P, WB ; Human . PubMed: 28826504
- Mashukova A et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci 127:3568-77 (2014). PubMed: 24876225
- Anderson LR et al. BAG-1 overexpression attenuates luminal apoptosis in MCF-10A mammary epithelial cells through enhanced RAF-1 activation. Oncogene 29:527-38 (2010). ICC/IF ; Human . PubMed: 19881545
