Recombinant
Anti-Bag1 antibody [Y166] (ab32109)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-Bag1 antibody [Y166]
See all Bag1 primary antibodies -
DescriptionRabbit monoclonal [Y166] to Bag1
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Host speciesRabbit
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SpecificityThe antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa cell lysate and cells, human prostate carcinoma tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
ClonalityMonoclonal
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Clone numberY166
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/80. |
Application notes
Is unsuitable for Flow Cyt.
Target
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FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
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Tissue specificityIsoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
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Sequence similaritiesContains 1 BAG domain.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localizationCytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
- Information by UniProt
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Database links
- Entrez Gene: 573 Human
- Entrez Gene: 12017 Mouse
- Entrez Gene: 297994 Rat
- Omim: 601497 Human
- SwissProt: Q99933 Human
- SwissProt: Q60739 Mouse
- SwissProt: B0K019 Rat
- Unigene: 377484 Human
see all -
Alternative names
- Bag 1 antibody
- BAG family molecular chaperone regulator 1 antibody
- BAG-1 antibody
see all
Images
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![Western blot - Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109.gif)
Western blot - Anti-Bag1 antibody [Y166] (ab32109)Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate
Predicted band size: 31 kDa
Observed band size: 33 kDa (why is the actual band size different from the predicted?)
Additional bands at: 46 kDa (possible isoform), 50 kDa (possible isoform) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)](https://www.abcam.com/ps/products/32/ab32109/Images/ab32109if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.
References
This product has been referenced in:
- Wood RJ et al. A biosensor-based framework to measure latent proteostasis capacity. Nat Commun 9:287 (2018). Read more (PubMed: 29348634) »
- Cato L et al. Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer. Elife 6:N/A (2017). IHC-P, WB ; Human . Read more (PubMed: 28826504) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"