Overview

  • Product name

    Anti-Bag1 antibody [Y166]
    See all Bag1 primary antibodies
  • Description

    Rabbit monoclonal [Y166] to Bag1
  • Host species

    Rabbit
  • Specificity

    The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate and cells, human prostate carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IP 1/80.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
    • Tissue specificity

      Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
    • Sequence similarities

      Contains 1 BAG domain.
      Contains 1 ubiquitin-like domain.
    • Post-translational
      modifications

      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • Cellular localization

      Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
    • Information by UniProt
    • Database links

    • Alternative names

      • Bag 1 antibody
      • BAG family molecular chaperone regulator 1 antibody
      • BAG-1 antibody
      • BAG1 antibody
      • BAG1_HUMAN antibody
      • BCL 2 Associated Athanogene 1 antibody
      • BCL 2 associated athanogene antibody
      • BCL 2 binding athanogene 1 antibody
      • Bcl-2-associated athanogene 1 antibody
      • BCL2 associated athanogene 1 antibody
      • BCL2 associated athanogene antibody
      • Glucocorticoid receptor-associated protein RAP46 antibody
      • HAP1 antibody
      • Haptoglobin antibody
      • RAP 46 antibody
      • RAP46 antibody
      see all

    Images

    • Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate

      Predicted band size: 31 kDa
      Observed band size: 33 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 46 kDa (possible isoform), 50 kDa (possible isoform)

    • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.

    References

    This product has been referenced in:

    • Gennaro VJ  et al. Interaction between the BAG1S isoform and HSP70 mediates the stability of anti-apoptotic proteins and the survival of osteosarcoma cells expressing oncogenic MYC. BMC Cancer 19:258 (2019). Read more (PubMed: 30902071) »
    • Wood RJ  et al. A biosensor-based framework to measure latent proteostasis capacity. Nat Commun 9:287 (2018). Read more (PubMed: 29348634) »
    See all 5 Publications for this product

    Customer reviews and Q&As

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    1-4 of 4 Abreviews

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample
    Human Cell (Human primary fibroblasts)
    Specification
    Human primary fibroblasts
    Permeabilization
    Yes - 0.2% Triton-X100
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 11 2013

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount
    20 µg
    Specification
    Mouse embryonic fibroblasts
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Nov 23 2011

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    200 µg
    Specification
    HeLa cells
    Immuno-precipitation step
    Protein A/G

    Abcam user community

    Verified customer

    Submitted Apr 06 2011

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

    Dr. Svetlana Khoronenkova

    Verified customer

    Submitted Aug 25 2010

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