Recombinant
RabMAb

Recombinant Anti-Bag1 antibody [Y166] - BSA and Azide free (ab211754)

Overview

  • Product name

    Anti-Bag1 antibody [Y166] - BSA and Azide free
    See all Bag1 primary antibodies
  • Description

    Rabbit monoclonal [Y166] to Bag1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate and cells, human prostate carcinoma tissue
  • General notes

    Ab211754 is the carrier-free version of ab32109. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab211754 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
    • Tissue specificity

      Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
    • Sequence similarities

      Contains 1 BAG domain.
      Contains 1 ubiquitin-like domain.
    • Post-translational
      modifications

      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • Cellular localization

      Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
    • Information by UniProt
    • Database links

    • Alternative names

      • Bag 1 antibody
      • BAG family molecular chaperone regulator 1 antibody
      • BAG-1 antibody
      • BAG1 antibody
      • BAG1_HUMAN antibody
      • BCL 2 Associated Athanogene 1 antibody
      • BCL 2 associated athanogene antibody
      • BCL 2 binding athanogene 1 antibody
      • Bcl-2-associated athanogene 1 antibody
      • BCL2 associated athanogene 1 antibody
      • BCL2 associated athanogene antibody
      • Glucocorticoid receptor-associated protein RAP46 antibody
      • HAP1 antibody
      • Haptoglobin antibody
      • RAP 46 antibody
      • RAP46 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32109).

    • Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32109).

    References

    This product has been referenced in:

    • Anderson LR  et al. BAG-1 overexpression attenuates luminal apoptosis in MCF-10A mammary epithelial cells through enhanced RAF-1 activation. Oncogene 29:527-38 (2010). ICC/IF ; Human . Read more (PubMed: 19881545) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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