Recombinant Anti-BAG2 antibody [EPR3567] (ab79406)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-BAG2 antibody [EPR3567]
See all BAG2 primary antibodies -
Description
Rabbit monoclonal [EPR3567] to BAG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BAG2 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa and HepG2 cell lysates; human ovarian carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3567 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). | |
| IP | 1/30. 1/30 refers to a volume/volume ratio of antibody as supplied and lysate. Optimization of the ratio may be necessary. |
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| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. -
Sequence similarities
Contains 1 BAG domain. - Information by UniProt
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Database links
- Entrez Gene: 9532 Human
- Omim: 603882 Human
- SwissProt: O95816 Human
- Unigene: 745046 Human
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Alternative names
- BAG 2 antibody
- BAG family molecular chaperone regulator 2 antibody
- BAG-2 antibody
see all
Images
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![Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-94961-anti-bag2-antibody-epr3567-western-blot.jpg)
Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/ab79406-94960-anti-bag2-antibody-epr3567-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Azoitei N et al. Targeting of KRAS mutant tumors by HSP90 inhibitors involves degradation of STK33. J Exp Med 209:697-711 (2012). WB . Read more (PubMed: 22451720) »
