Anti-BAG2 antibody [EPR3567] (ab79406)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-BAG2 antibody [EPR3567]
See all BAG2 primary antibodies -
DescriptionRabbit monoclonal [EPR3567] to BAG2
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human BAG2 aa 1-100 (N terminal). The exact sequence is proprietary.
-
Positive control
- Jurkat, HeLa and HepG2 cell lysates; human ovarian carcinoma tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR3567
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab79406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). | |
| IP | 1/10. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
-
Sequence similaritiesContains 1 BAG domain.
- Information by UniProt
-
Database links
- Entrez Gene: 9532 Human
- Omim: 603882 Human
- SwissProt: O95816 Human
- Unigene: 745046 Human
-
Alternative names
- BAG 2 antibody
- BAG family molecular chaperone regulator 2 antibody
- BAG-2 antibody
see all
Images
-
![Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/BAG2-Primary-antibodies-ab79406-1.jpg)
Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)](https://www.abcam.com/ps/products/79/ab79406/Images/BAG2-Primary-antibodies-ab79406-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Protocols
References
This product has been referenced in:
- Azoitei N et al. Targeting of KRAS mutant tumors by HSP90 inhibitors involves degradation of STK33. J Exp Med 209:697-711 (2012). WB . Read more (PubMed: 22451720) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"