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Cell Biology Apoptosis Intracellular Bcl2 Family
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
  • Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3567] to BAG2 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-BAG2 antibody [EPR3567] - BSA and Azide free
    See all BAG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3567] to BAG2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and HeLa cell lysates.
  • General notes

    ab247500 is the carrier-free version of ab79406 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247500 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3567
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-BAG2 antibody [EPR3567] (ab79406)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • KO cell lines

    • Human BAG2 knockout HeLa cell line (ab265907)
  • KO cell lysates

    • Human BAG2 knockout HeLa cell lysate (ab257369)
  • Recombinant Protein

    • Recombinant Human BAG2 protein (ab104795)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
  • Sequence similarities

    Contains 1 BAG domain.
  • Target information above from: UniProt accession O95816 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9532 Human
    • Omim: 603882 Human
    • SwissProt: O95816 Human
    • Unigene: 745046 Human
    • Alternative names

      • BAG 2 antibody
      • BAG family molecular chaperone regulator 2 antibody
      • BAG-2 antibody
      • BAG-family molecular chaperone regulator-2 antibody
      • Bag2 antibody
      • BAG2_HUMAN antibody
      • BCL 2 associated athanogene 2 antibody
      • Bcl-2-associated athanogene 2 antibody
      • BCL2 associated athanogene 2 antibody
      • BCL2-associated athanogene 2 antibody
      • dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody
      • dJ417I1.2 antibody
      • KIAA0576 antibody
      • MGC149462 antibody
      • OTTHUMP00000016668 antibody
      see all

    Images

    • Western blot - Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
      Western blot - Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
      All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : BAG2 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 24 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation (ab79406).

      Lanes 1-3: Merged signal (red and green). Green - ab79406 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab79406 Anti-BAG2 antibody [EPR3567] was shown to specifically react with BAG2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265907 (knockout cell lysate ab257369) was used.  Wild-type and BAG2 knockout samples were subjected to SDS-PAGE.  ab79406 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
      Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab247500? Please let us know so that we can cite the reference in this datasheet.

    ab247500 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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