Recombinant Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
![Western blot - Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)](https://www.abcam.com/ps/products/247/ab247500/Images/ab247500-393674-anti-bag2-antibody-epr3567-western-blot-human-knockout-lysate.jpg "Western blot - Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3567] to BAG2 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-BAG2 antibody [EPR3567] - BSA and Azide free
See all BAG2 primary antibodies -
Description
Rabbit monoclonal [EPR3567] to BAG2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and HeLa cell lysates.
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General notes
ab247500 is the carrier-free version of ab79406 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247500 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3567 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. -
Sequence similarities
Contains 1 BAG domain. - Information by UniProt
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Database links
- Entrez Gene: 9532 Human
- Omim: 603882 Human
- SwissProt: O95816 Human
- Unigene: 745046 Human
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Alternative names
- BAG 2 antibody
- BAG family molecular chaperone regulator 2 antibody
- BAG-2 antibody
see all
Images
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Western blot - Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BAG2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab79406).
Lanes 1-3: Merged signal (red and green). Green - ab79406 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.
ab79406 Anti-BAG2 antibody [EPR3567] was shown to specifically react with BAG2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265907 (knockout cell lysate ab257369) was used. Wild-type and BAG2 knockout samples were subjected to SDS-PAGE. ab79406 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)](https://www.abcam.com/ps/products/247/ab247500/Images/ab247500-2-benefits-of-recombinant-antibodies.png)
Anti-BAG2 antibody [EPR3567] - BSA and Azide free (ab247500)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab247500 has not yet been referenced specifically in any publications.