Key features and details
- Rabbit polyclonal to Bag3
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bag3 antibody
See all Bag3 primary antibodies
DescriptionRabbit polyclonal to Bag3
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Bag3 aa 525-575. The exact sequence is proprietary. (NP_004272.2).
Database link: O95817
- IHC-P: Human lung carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225561 was affinity purified using an epitope specific to Bag3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 62 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in diseaseDefects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
Sequence similaritiesContains 1 BAG domain.
Contains 2 WW domains.
- Information by UniProt
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
All lanes : Anti-Bag3 antibody (ab225561) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Bag3 using ab225561 at 1/500 dilution in immunohistochemical analysis. Detection: DAB staining.
Bag3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225561 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225561 at 0.4 µg/ml.
Lane 1: ab225561 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225561 has not yet been referenced specifically in any publications.