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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-Bag3 antibody (ab225561)

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Western blot - Anti-Bag3 antibody (ab225561)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (ab225561)
  • Immunoprecipitation - Anti-Bag3 antibody (ab225561)

Key features and details

  • Rabbit polyclonal to Bag3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human Bag3 protein (ab95384)

View more associated products

Overview

  • Product name

    Anti-Bag3 antibody
    See all Bag3 primary antibodies
  • Description

    Rabbit polyclonal to Bag3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Bag3 aa 525-575. The exact sequence is proprietary. (NP_004272.2).
    Database link: O95817

  • Positive control

    • IHC-P: Human lung carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225561 was affinity purified using an epitope specific to Bag3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Bag3 protein (ab95384)
  • Related Products

    • Recombinant Human Bag3 protein (ab95384)

Applications

Our Abpromise guarantee covers the use of ab225561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • Involvement in disease

    Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • Sequence similarities

    Contains 1 BAG domain.
    Contains 2 WW domains.
  • Target information above from: UniProt accession O95817 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9531 Human
    • Omim: 603883 Human
    • SwissProt: O95817 Human
    • Unigene: 523309 Human
    • Alternative names

      • BAG 3 antibody
      • BAG family molecular chaperone regulator 3 antibody
      • BAG-3 antibody
      • Bag3 antibody
      • BAG3_HUMAN antibody
      • Bcl 2 binding protein antibody
      • Bcl-2-associated athanogene 3 antibody
      • Bcl-2-binding protein Bis antibody
      • BCL2 associated athanogene 3 antibody
      • BCL2 binding athanogene 3 antibody
      • BIS antibody
      • CAIR 1 antibody
      • Docking protein CAIR 1 antibody
      • Docking protein CAIR-1 antibody
      • MFM6 antibody
      see all

    Images

    • Western blot - Anti-Bag3 antibody (ab225561)
      Western blot - Anti-Bag3 antibody (ab225561)
      All lanes : Anti-Bag3 antibody (ab225561) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 62 kDa


      Exposure time: 30 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (ab225561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (ab225561)

      Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Bag3 using ab225561 at 1/500 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Immunoprecipitation - Anti-Bag3 antibody (ab225561)
      Immunoprecipitation - Anti-Bag3 antibody (ab225561)

      Bag3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225561 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225561 at 0.4 µg/ml.

      Lane 1: ab225561 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 3 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab225561? Please let us know so that we can cite the reference in this datasheet.

    ab225561 has not yet been referenced specifically in any publications.

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