• Product name

  • Description

    Rabbit polyclonal to Bag3
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.

  • Positive control

    • Muscle, PANC-1 pancreatic cell line (ATCC-CRL 1469)



Our Abpromise guarantee covers the use of ab47124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 62 kDa.
IP 1/50 - 1/200.


  • Function

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • Involvement in disease

    Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • Sequence similarities

    Contains 1 BAG domain.
    Contains 2 WW domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAG 3 antibody
    • BAG family molecular chaperone regulator 3 antibody
    • BAG-3 antibody
    • Bag3 antibody
    • BAG3_HUMAN antibody
    • Bcl 2 binding protein antibody
    • Bcl-2-associated athanogene 3 antibody
    • Bcl-2-binding protein Bis antibody
    • BCL2 associated athanogene 3 antibody
    • BCL2 binding athanogene 3 antibody
    • BIS antibody
    • CAIR 1 antibody
    • Docking protein CAIR 1 antibody
    • Docking protein CAIR-1 antibody
    • MFM6 antibody
    see all


  • All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution

    Lane 1 : Thymus lysates, total protein (20ug/lane)
    Lane 2 : Spleen lysates, total protein (20ug/lane)
    Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
    Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
    Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)

    Predicted band size: 62 kDa
    Observed band size: 86 kDa
    why is the actual band size different from the predicted?

    Tissue lysates, normalized for total protein (20 µg/lane), were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
  • ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."
  • Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
  • Anti-Bag3 antibody (ab47124) + Hela lysate at 0.5 mg/ml

    Predicted band size: 62 kDa

  • Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).


This product has been referenced in:

  • Lu SZ  et al. Suppression of astrocytic autophagy by aB-crystallin contributes to a-synuclein inclusion formation. Transl Neurodegener 8:3 (2019). Read more (PubMed: 30675347) »
  • Seminary ER  et al. Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons. Front Neurosci 12:86 (2018). Read more (PubMed: 29515358) »
See all 13 Publications for this product

Customer reviews and Q&As


Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

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