Key features and details
- Rabbit polyclonal to Bag3
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Bag3 antibody
See all Bag3 primary antibodies
DescriptionRabbit polyclonal to Bag3
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Human
A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab47124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/1000 - 1/5000.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 62 kDa.|
FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in diseaseDefects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
Sequence similaritiesContains 1 BAG domain.
Contains 2 WW domains.
- Information by UniProt
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size: 62 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
Tissue lysates, normalized for total protein (20 µg/lane), were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."
Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
Anti-Bag3 antibody (ab47124) + Hela lysate at 0.5 mg/ml
Predicted band size: 62 kDa
Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
ab47124 has been referenced in 17 publications.
- Lu SZ et al. Suppression of astrocytic autophagy by aB-crystallin contributes to a-synuclein inclusion formation. Transl Neurodegener 8:3 (2019). PubMed: 30675347
- Sun H et al. FAM111B, a direct target of p53, promotes the malignant process of lung adenocarcinoma. Onco Targets Ther 12:2829-2842 (2019). PubMed: 31114230
- Al-Tahan S et al. New family with HSPB8-associated autosomal dominant rimmed vacuolar myopathy. Neurol Genet 5:e349 (2019). PubMed: 31403083
- McDermott-Roe C et al. Investigation of a dilated cardiomyopathy-associated variant in BAG3 using genome-edited iPSC-derived cardiomyocytes. JCI Insight 4:N/A (2019). PubMed: 31723063
- Haidar M et al. Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies. Autophagy 15:1051-1068 (2019). PubMed: 30669930