Overview

  • Product name
  • Description
    Goat polyclonal to Bag3
  • Host species
    Goat
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: SSMTDTPGNPAAP, corresponding to C terminal amino acids 563-575 of Human Bag3.

  • Positive control
    • Recombinant Human Bag3 protein (ab95384) can be used as a positive control in WB. Human skeletal muscle lysates.
  • General notes
    GenBank Accession Number – NP_004272

Properties

Applications

Our Abpromise guarantee covers the use of ab5898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 23341456
WB Use a concentration of 0.1 - 0.3 µg/ml. Can be blocked with Human Bag3 peptide (ab23111).
Approx 85kDa band observed in Human skeletal muscle lysates (calculated MW of 61.6kDa according to NP_004272.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Iwasaki et al, Cancer Res. 2007 Nov 1;67(21):10252-9. PMID: 17974966).

Target

  • Function
    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • Involvement in disease
    Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • Sequence similarities
    Contains 1 BAG domain.
    Contains 2 WW domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG 3 antibody
    • BAG family molecular chaperone regulator 3 antibody
    • BAG-3 antibody
    • Bag3 antibody
    • BAG3_HUMAN antibody
    • Bcl 2 binding protein antibody
    • Bcl-2-associated athanogene 3 antibody
    • Bcl-2-binding protein Bis antibody
    • BCL2 associated athanogene 3 antibody
    • BCL2 binding athanogene 3 antibody
    • BIS antibody
    • CAIR 1 antibody
    • Docking protein CAIR 1 antibody
    • Docking protein CAIR-1 antibody
    • MFM6 antibody
    see all

References

This product has been referenced in:
  • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). IP ; Human . Read more (PubMed: 23341456) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for providing more information about your protocol. We would like to emphasize that this is a Fast-Track antibody and not fully characterized yet. Please take a look at the Terms and Conditions for Fast-Track products. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price Our preliminary results show an approx 80 kDa band in HeLa and 293 lysates at 1ug of primary on Western blot application. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 67Kda according to NP_004272. In order to be able to reduce the high background, we would suggest loading 20 or 30 ug protein per lane and/or dilute the antibody a bit further down (i.e. 1/500 or 1/1000). We would suggest perhaps submitting a review on-line. In return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. We hope this will help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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