Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20207-109-3] to Bag3
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Bag3 antibody [EPR20207-109-3]
See all Bag3 primary antibodies
DescriptionRabbit monoclonal [EPR20207-109-3] to Bag3
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Bag3 aa 1-250. The exact sequence is proprietary.
Database link: O95817
- WB: Human skeletal muscle lysate. Mouse and rat heart lysate. HeLa whole cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).|
FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in diseaseDefects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
Sequence similaritiesContains 1 BAG domain.
Contains 2 WW domains.
- Information by UniProt
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Bag3 with ab209251 at 1/60 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line. The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The control is the secondary antibody only.
Negative control cell line: HL-60 (PMID: 17974966).
All lanes : Anti-Bag3 antibody [EPR20207-109-3] (ab209251) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat heart lysate
Lane 5 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 62 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17974966).
Negative control: HL-60 (PMID: 17974966).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 60 seconds; Lane 3: 30 seconds; Lane 4: 10 seconds; Lane 5: 180 seconds.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HL-60 (human promyelocytic leukemia cell line, Left panel) HeLa (human cervix adenocarcinoma epithelial cell, Right panel) labeling Bag3 with ab209251 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: HL-60 (PMID: 17974966).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab209251 has not yet been referenced specifically in any publications.