Recombinant
RabMAb

Recombinant Anti-Bag3 antibody [EPR20207-109-3] (ab209251)

Overview

  • Product name

    Anti-Bag3 antibody [EPR20207-109-3]
    See all Bag3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20207-109-3] to Bag3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Bag3 aa 1-250. The exact sequence is proprietary.
    Database link: O95817

  • Positive control

    • WB: Human skeletal muscle lysate. Mouse and rat heart lysate. HeLa whole cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
ICC/IF 1/60.
Flow Cyt 1/60.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
    • Involvement in disease

      Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
    • Sequence similarities

      Contains 1 BAG domain.
      Contains 2 WW domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAG 3 antibody
      • BAG family molecular chaperone regulator 3 antibody
      • BAG-3 antibody
      • Bag3 antibody
      • BAG3_HUMAN antibody
      • Bcl 2 binding protein antibody
      • Bcl-2-associated athanogene 3 antibody
      • Bcl-2-binding protein Bis antibody
      • BCL2 associated athanogene 3 antibody
      • BCL2 binding athanogene 3 antibody
      • BIS antibody
      • CAIR 1 antibody
      • Docking protein CAIR 1 antibody
      • Docking protein CAIR-1 antibody
      • MFM6 antibody
      see all

    Images

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Bag3 with ab209251 at 1/60 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line. The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The control is the secondary antibody only.

      Negative control cell line: HL-60 (PMID: 17974966).

    • All lanes : Anti-Bag3 antibody [EPR20207-109-3] (ab209251) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : Mouse heart lysate
      Lane 4 : Rat heart lysate
      Lane 5 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 62 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?



      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17974966).

      Negative control: HL-60 (PMID: 17974966).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lanes 1-2: 60 seconds; Lane 3: 30 seconds; Lane 4: 10 seconds; Lane 5: 180 seconds.

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HL-60 (human promyelocytic leukemia cell line, Left panel) HeLa (human cervix adenocarcinoma epithelial cell, Right panel) labeling Bag3 with ab209251 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Negative control: HL-60 (PMID: 17974966).

    References

    ab209251 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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