Recombinant Anti-Bag3 antibody [EPR3515] (ab92309)
![Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)](https://www.abcam.com/ps/products/92/ab92309/Images/ab92309-293351-anti-bag3-antibody-epr3515-western-blot.jpg "Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3515] to Bag3
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bag3 antibody [EPR3515]
See all Bag3 primary antibodies -
Description
Rabbit monoclonal [EPR3515] to Bag3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Bag3 aa 50-150. The exact sequence is proprietary.
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Positive control
- 293T, HeLa, MCF7 and K562 cell lysates. IP: Mouse heart lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3515 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa). |
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IP
1/10 - 1/100. |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
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Database links
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 523309 Human
- Unigene: 84073 Mouse
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Alternative names
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
Images
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Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BAG3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92309 was shown to recognize BAG3 in wild-type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. Ab92309 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (ab92309)](https://www.abcam.com/ps/products/92/ab92309/Images/ab92309-4-anti-bag3-antibody-epr3515-immunoprecipitation-heart-mouse.jpg)
Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (ab92309)Purified ab92309 at 1/50 dilution (2µg) immunoprecipitating Bag3 in Mouse heart lysate.
Lane 1 (input): Mouse heart lysate 10µg
Lane 2 (+): ab92309 + Mouse heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92309 in Mouse heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 80 kDa -
![Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)](https://www.abcam.com/ps/products/92/ab92309/Images/ab92309-107241-anti-bag3-antibody-epr3515-western-blot.jpg)
Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)All lanes : Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution
Lane 1 : 293T cell
lysate
Lane 2 : HeLa cell
lysate
Lane 3 :
MCF7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Anti-Bag3 antibody [EPR3515] (ab92309)](https://www.abcam.com/ps/products/92/ab92309/Images/ab92309-3-benefits-of-recombinant-antibodies.png)
Anti-Bag3 antibody [EPR3515] (ab92309)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab92309 has been referenced in 7 publications.
- Abe S et al. ERa increases endometrial cancer cell resistance to cisplatin via upregulation of BAG3. Oncol Lett 21:20 (2021). PubMed: 33240426
- Pataer A et al. Therapeutic targeting of the PI4K2A/PKR lysosome network is critical for misfolded protein clearance and survival in cancer cells. Oncogene 39:801-813 (2020). PubMed: 31554935
- Yuan T et al. Inhibition of the PI3K/AKT signaling pathway sensitizes diffuse large B-cell lymphoma cells to treatment with proteasome inhibitors via suppression of BAG3. Oncol Lett 17:3719-3726 (2019). PubMed: 30881494
- Shields S et al. BAG3 promotes tumour cell proliferation by regulating EGFR signal transduction pathways in triple negative breast cancer. Oncotarget 9:15673-15690 (2018). PubMed: 29644001
- Qi J et al. The role of Bag2 in neurotoxicity induced by the anesthetic sevoflurane. J Cell Biochem N/A:N/A (2018). PubMed: 30548665
- Yunoki T et al. Expression of Anti-apoptotic Protein BAG3 in Human Sebaceous Gland Carcinoma of the Eyelid. Anticancer Res 37:1931-1934 (2017). PubMed: 28373462
- Xiao H et al. BAG3 and HIF-1 a Coexpression Detected by Immunohistochemistry Correlated with Prognosis in Hepatocellular Carcinoma after Liver Transplantation. Biomed Res Int 2014:516518 (2014). WB, IHC-P ; Human . PubMed: 24895585