Recombinant Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)
![Western blot - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)](https://www.abcam.com/ps/products/247/ab247535/Images/ab247535-403260-anti-bag3-antibody-epr3515-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3515] to Bag3 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bag3 antibody [EPR3515] - BSA and Azide free
See all Bag3 primary antibodies -
Description
Rabbit monoclonal [EPR3515] to Bag3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Mouse heart lysate.
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General notes
ab247535 is the carrier-free version of ab92309.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3515 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
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Database links
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 523309 Human
- Unigene: 84073 Mouse
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Alternative names
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
Images
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Western blot - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)
This data was developed using ab92309, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BAG3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92309 was shown to recognize BAG3 in wild-type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. Ab92309 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)](https://www.abcam.com/ps/products/247/ab247535/Images/ab247535-397985-anti-bag3-antibody-epr3515-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)All lanes : Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?This data was developed using ab92309, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)](https://www.abcam.com/ps/products/247/ab247535/Images/ab247535-4-anti-bag3-antibody-epr3515-immunoprecipitation-heart-mouse.jpg)
Immunoprecipitation - Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)This data was developed using ab92309, the same antibody clone in a different buffer formulation.
Purified ab92309 at 1/50 dilution (2µg) immunoprecipitating Bag3 in Mouse heart lysate.
Lane 1 (input): Mouse heart lysate 10µg
Lane 2 (+): ab92309 + Mouse heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92309 in Mouse heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 80 kDa -
![Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)](https://www.abcam.com/ps/products/247/ab247535/Images/ab247535-3-benefits-of-recombinant-antibodies.png)
Anti-Bag3 antibody [EPR3515] - BSA and Azide free (ab247535)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247535 has not yet been referenced specifically in any publications.
