Key features and details
- Rabbit polyclonal to Bag5
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bag5 antibody
See all Bag5 primary antibodies
DescriptionRabbit polyclonal to Bag5
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment, corresponding to a sequence within amino acids 1-165 of Human Bag5 (NP_004864).
- 293T, HeLa, MOLT4 and Raji cell lysates; A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionInhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
Sequence similaritiesContains 5 BAG domains.
DomainThe fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
- Information by UniProt
- BAG family molecular chaperone regulator 5 antibody
- BAG-5 antibody
- BAG5 antibody
All lanes : Anti-Bag5 antibody (ab97660) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
ab97660 at 1/200 dilution staining Bag5 in A549 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
ab97660 has been referenced in 2 publications.
- Xi J et al. miR-155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling. Mol Med Rep 21:1011-1020 (2020). PubMed: 31922242
- Bruchmann A et al. Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis. BMC Cancer 13:96 (2013). WB, ICC/IF ; Human . PubMed: 23448667