Product nameBag5 overexpression 293T lysate (whole cell)
DescriptionBag5 overexpression lysate
ab94322 is a 293T cell transfected lysate in which Human Bag5 has been transiently over-expressed using a pCMV-Bag5 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. Similarity: Contains 5 BAG domains. Domain: The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
Our Abpromise guarantee covers the use of ab94322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94322 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Bag5 antibody (ab88100) at 1/500 dilution
Lane 1 :
Bag5 overexpression 293T lysate (whole cell) (ab94322)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94322 has not yet been referenced specifically in any publications.