Product nameAnti-BAHD1 antibody
See all BAHD1 primary antibodies
Predicted to work with: Mouse, Human
- This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat and HEK293
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Optimal dilutions/concentrations should be determined by the end user.
RelevanceBAHD1 (bromo adjacent homology domain-containing 1 protein) is, as its name suggests, a bromo-adjacent homology (BAH) domain. The BAH domain is found in eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, and several proteins involved in transcriptional regulation. It is thought to function as a protein-protein interaction domain. The BAH domain of yeast Sir3p has been shown to bind to nucleosomes and to the N-terminal tail of histone H3.
Cellular localizationNucleus. Note: Localizes to heterochromatin and inactive X chromosome. Colocalizes with histone H3 trimethylated at 'Lys-27' (H3K27me3).
- AL022997 antibody
- AW541238 antibody
- BAH domain containing protein 1 antibody
All lanes : Anti-BAHD1 antibody (ab46573) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab46573 detects a band at 75 kDa in a range of human cell lysates. We are unsure as to why the band seen is lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.
This product has been referenced in:
- Libertini E et al. Overexpression of the Heterochromatinization Factor BAHD1 in HEK293 Cells Differentially Reshapes the DNA Methylome on Autosomes and X Chromosome. Front Genet 6:339 (2015). WB, ICC/IF ; Human . Read more (PubMed: 26648976) »