Overview

  • Product name
  • Description
    Rabbit polyclonal to BAI1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human BAI1 aa 683-732 (internal sequence).

  • Positive control
    • HUVEC cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ or Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit anti-serum by immunogenic peptide affinity-chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24509909
WB 1/500 - 1/1000. Predicted molecular weight: 174 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Likely to be a potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53 signal in suppression of glioblastoma. May function in cell adhesion and signal transduction in the brain.
  • Tissue specificity
    Specifically expressed in brain. Reduced or no expression is observed in some glioblastoma cell lines and cancer tissues. No expression in astrocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.
    Contains 5 TSP type-1 domains.
  • Domain
    The TSP1 repeats inhibit in vivo angiogenesis in rat cornea induced by BFGF.
  • Post-translational
    modifications
    The endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.
  • Cellular localization
    Cell membrane. Likely to be concentrated at cell-cell adhesion sites.
  • Information by UniProt
  • Database links
  • Alternative names
    • B830018M07Rik antibody
    • BAI 1 antibody
    • BAI1 antibody
    • BAI1_HUMAN antibody
    • Brain specific angiogenesis inhibitor 1 antibody
    • Brain-specific angiogenesis inhibitor 1 antibody
    • GDAIF antibody
    • KIAA4089 antibody
    • mKIAA4089 antibody
    • R75078 antibody
    see all

Images

  • Anti-BAI1 antibody (ab135907) at 1/500 dilution + Huvec cell lysate at 30 µg

    Predicted band size: 174 kDa

  • Immunohistochemical analysis of paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized paraffin-embedded mouse hippocampus tissue labeling BAI1 with ab135907 at 1/50 dilution,followed by Goat Anti-Rabbit IgG (HRP) at 1/500 dilution.

References

This product has been referenced in:
  • Tian DW  et al. Biological effects of eukaryotic recombinant plasmid pReceiver-M61-BAI-1 transfection on T24 cells and HUVECs. Mol Med Rep 14:1553-9 (2016). Human . Read more (PubMed: 27356780) »
  • Belone Ade F  et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . Read more (PubMed: 26635870) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
Sample
Mouse Tissue sections (mouse hippocampus)
Specification
mouse hippocampus
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 23 2015

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7% Gel)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Treatment
hBAI1-cDNA overexpressed
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 05 2013

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