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Anti-BAI3 antibody - C-terminal (ab189121)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAI3 antibody - C-terminal (ab189121)

    Key features and details

    • Rabbit polyclonal to BAI3 - C-terminal
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Recombinant Human BAI3 protein (ab151879)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-BAI3 antibody - C-terminal
      See all BAI3 primary antibodies
    • Description

      Rabbit polyclonal to BAI3 - C-terminal
    • Host species

      Rabbit
    • Specificity

      BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human BAI3 (C terminal). (19 amino acid peptide; NP_001695.1).
      Database link: O60242

    • Positive control

      • Human brain, neurons.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Inhibitors
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human BAI3 protein (ab151879)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab189121 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    All applications
    Mouse
    Rat
    Rabbit
    Horse
    Chicken
    Cow
    Dog
    Monkey
    Gorilla
    Application Abreviews Notes
    IHC-P
    Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    • Function

      Might be involved in angiogenesis inhibition and suppression of glioblastoma.
    • Tissue specificity

      Strongly expressed in brain. Also detected in heart. Reduced expression is observed in some glioblastoma cell lines.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 1 CUB domain.
      Contains 1 GPS domain.
      Contains 4 TSP type-1 domains.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession O60242 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 577 Human
      • Entrez Gene: 210933 Mouse
      • Entrez Gene: 301309 Rat
      • Omim: 602684 Human
      • SwissProt: O60242 Human
      • SwissProt: Q80ZF8 Mouse
      • Unigene: 13261 Human
      • Unigene: 336569 Mouse
      • Unigene: 204758 Rat
      see all
    • Alternative names

      • BAI3 antibody
      • BAI3_HUMAN antibody
      • Brain-specific angiogenesis inhibitor 3 antibody
      • dJ91B17.1 (brain specific angiogenesis inhibitor 3) antibody
      • KIAA0550 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAI3 antibody - C-terminal (ab189121)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAI3 antibody - C-terminal (ab189121)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain, neurons labeling BAI3 with ab189121 at 4 µg/ml.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab189121? Please let us know so that we can cite the reference in this datasheet.

    ab189121 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-BAI3 antibody - C-terminal

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Medulloblastoma)
    Gel Running Conditions
    Non-reduced Denaturing (8%SDS-PAGE)
    Loading amount
    20 µg
    Specification
    Medulloblastoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Chaoxi Li

    Verified customer

    Submitted Aug 13 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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