Application
Western blot
Sample
Human Cell lysate - whole cell (Medulloblastoma)
Gel Running Conditions
Non-reduced Denaturing (8%SDS-PAGE)
Loading amount
20 µg
Specification
Medulloblastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Rabbit IgG HRP Linked Whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
no
Exposure
20 minute(s) and 0 second(s)
Positive control
see our image
Additional data
Abcam response
Abcam has not tested ab189121 in western blot and thus could not guarantee the antibody performance in application. The researcher received a credit for testing the antibody in western blot as part of our AbTrial program.
Chaoxi Li
Verified customer
Submitted Aug 13 2020