Overview

  • Product name

    Anti-BAIAP2L1/IRTKS antibody
    See all BAIAP2L1/IRTKS primary antibodies
  • Description

    Rabbit polyclonal to BAIAP2L1/IRTKS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human BAIAP2L1/IRTKS aa 461-511. The exact sequence is proprietary.
    Database link: Q9UHR4

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as BAIAP2L1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226344 was affinity purified using an epitope specific to BAIAP2L1/IRTKS immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.
  • Sequence similarities

    Contains 1 IMD (IRSp53/MIM homology) domain.
    Contains 1 SH3 domain.
  • Domain

    The IMD domain is predicted to have a helical structure. It may induce actin bundling and filopodia formation.
  • Post-translational
    modifications

    Phosphorylated on tyrosine in response to insulin.
  • Cellular localization

    Cytoplasm, cytoskeleton. Recruited to actin pedestals that are formed upon infection by bacteria at bacterial attachment sites.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAI1 associated protein 2 like 1 antibody
    • BAI1 associated protein 2 like protein 1 antibody
    • BAI1-associated protein 2-like protein 1 antibody
    • Baiap2l1 antibody
    • BI2L1_HUMAN antibody
    • Brain specific angiogenesis inhibitor 1 associated protein 2 like protein 1 antibody
    • Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1 antibody
    • FLJ42275 antibody
    • Insulin receptor tyrosine kinase substrate antibody
    • IRTKS antibody
    see all

Images

  • All lanes : Anti-BAIAP2L1/IRTKS antibody (ab226344) at 0.1 mg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa


    Exposure time: 30 seconds


    Cells prepared using NETN lysis buffer.

  • BAIAP2L1/IRTKS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226344 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226344 at 1 µg/ml.

    Lane 1: ab226344 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Cells prepared using NETN lysis buffer.

References

ab226344 has not yet been referenced specifically in any publications.

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