Anti-BAIAP2L1/IRTKS antibody (ab251753)
Key features and details
- Rabbit polyclonal to BAIAP2L1/IRTKS
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-BAIAP2L1/IRTKS antibody
See all BAIAP2L1/IRTKS primary antibodies -
Description
Rabbit polyclonal to BAIAP2L1/IRTKS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BAIAP2L1/IRTKS aa 400 to the C-terminus.
Database link: Q9UHR4 -
Positive control
- IHC-P: Human duodenum and skeletal muscle tissues. ICC/IF: U-2 OS cells. WB: Caco-2 and RT4 whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa.
|
|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. -
Sequence similarities
Contains 1 IMD (IRSp53/MIM homology) domain.
Contains 1 SH3 domain. -
Domain
The IMD domain is predicted to have a helical structure. It may induce actin bundling and filopodia formation. -
Post-translational
modificationsPhosphorylated on tyrosine in response to insulin. -
Cellular localization
Cytoplasm, cytoskeleton. Recruited to actin pedestals that are formed upon infection by bacteria at bacterial attachment sites. - Information by UniProt
-
Database links
- Entrez Gene: 55971 Human
- Omim: 611877 Human
- SwissProt: Q9UHR4 Human
- Unigene: 656063 Human
-
Alternative names
- BAI1 associated protein 2 like 1 antibody
- BAI1 associated protein 2 like protein 1 antibody
- BAI1-associated protein 2-like protein 1 antibody
see all
Images
-
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for BAIAP2L1/IRTKS (green) using ab251753 at 4 µg/ml in ICC/IF.
-
Paraffin-embedded human duodenum tissue stained for BAIAP2L1/IRTKS using ab251753 at 1/200 dilution in immunohistochemical analysis.
-
Paraffin-embedded human skeletal muscle tissue stained for BAIAP2L1/IRTKS using ab251753 at 1/200 dilution in immunohistochemical analysis.
-
All lanes : Anti-BAIAP2L1/IRTKS antibody (ab251753) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 57 kDa -
All lanes : Anti-BAIAP2L1/IRTKS antibody (ab251753) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 57 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab251753 has not yet been referenced specifically in any publications.