Key features and details
- Rabbit polyclonal to Bak
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Hamster, Human, Pig, Monkey
- Isotype: IgG
Product nameAnti-Bak antibody
See all Bak primary antibodies
DescriptionRabbit polyclonal to Bak
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Hamster, Human, Pig, Monkey
Synthetic peptide derived from the sequence of human Bak, conjugated to KLH.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionIn the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
Tissue specificityExpressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localizationMitochondrion membrane.
- Information by UniProt
- Apoptosis regulator BAK antibody
- BAK antibody
- BAK like antibody
Anti-Bak antibody (ab69404) at 1/1000 dilution + A431 cell lysate
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5.
Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.
ab69404 has been referenced in 3 publications.
- Feng Q et al. Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC Cancer 20:665 (2020). PubMed: 32677906
- Yuan C et al. BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells. Exp Ther Med 15:235-241 (2018). PubMed: 29375685
- Li Z et al. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4. Oncol Rep 38:2705-2716 (2017). PubMed: 29048629