Anti-Bak antibody [AT38E2] (ab104124)
Key features and details
- Mouse monoclonal [AT38E2] to Bak
- Suitable for: Flow Cyt, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Bak antibody [AT38E2]
See all Bak primary antibodies -
Description
Mouse monoclonal [AT38E2] to Bak -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to amino acids 29-187 of Human Bak, purified from E. coli.
-
Positive control
- WB: Mouse lung lysate, 293T, HeLa, A431, A549, Jurkat, MCF7, and PC3 cell lysates ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
-
General notes
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT38E2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
|
|
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 578 Human
- Entrez Gene: 12018 Mouse
- Omim: 600516 Human
- SwissProt: Q16611 Human
- SwissProt: O08734 Mouse
- Unigene: 485139 Human
- Unigene: 2443 Mouse
-
Alternative names
- Apoptosis regulator BAK antibody
- BAK antibody
- BAK like antibody
see all
Images
-
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BAK knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab104124 observed at 0 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab104124 was found to be non-specific when BAK knockout samples were used. Wild-type and BAK knockout samples were subjected to SDS-PAGE. Ab104124 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This image was produced using the ascites version of this antibody.
-
Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ab104124 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
-
Immunocytochemistry/ Immunofluorescence analysis of BAK in HeLa cells. The cell was stained with ab104124 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
-
All lanes : Anti-Bak antibody [AT38E2] (ab104124) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : PC3 cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 23 kDa -
Anti-Bak antibody [AT38E2] (ab104124) at 1/500 dilution + Mouse lung lyasate at 35 µg
Secondary
Goat anti-mouse HRP conjugate
Developed using the ECL technique.
Predicted band size: 23 kDaThis image was produced using the ascites version of this antibody.
-
Immunofluorescence staining of Bak in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104124 antibody (1/500) with Texas Red (Red).
This image was produced using the ascites version of this antibody.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab104124 has been referenced in 5 publications.
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Loo LSW et al. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis 11:378 (2020). PubMed: 32424151
- Singh A et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). PubMed: 28289414
- Liu P et al. Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1. Iran J Basic Med Sci 20:1260-1267 (2017). PubMed: 29299204
- Mebratu YA et al. Bik reduces hyperplastic cells by increasing Bak and activating DAPk1 to juxtapose ER and mitochondria. Nat Commun 8:803 (2017). PubMed: 28986568