Overview

  • Product name

    Anti-Bak antibody [NO.48A10]
    See all Bak primary antibodies
  • Description

    Rabbit monoclonal [NO.48A10] to Bak
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Bak aa 14-36.
    Sequence:

    CGEPALPSASEEQVAQDTEEVFR


    Database link: Q16611

  • Positive control

    • WB: HeLa and AGS cell extract. IHC-P: Mouse and human lung tissues. ICC/IF: CRC cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    NO.48A10
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
  • Tissue specificity

    Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis regulator BAK antibody
    • BAK antibody
    • BAK like antibody
    • Bak NT antibody
    • BAK_HUMAN antibody
    • Bak1 antibody
    • Bcl 2 homologous antagonist/killer antibody
    • Bcl 2 like 7 protein antibody
    • Bcl-2 homologous antagonist/killer antibody
    • Bcl-2-like protein 7 antibody
    • BCL2 antagonist/killer 1 antibody
    • Bcl2 like 7 Protein antibody
    • Bcl2-L-7 antibody
    • BCL2L7 antibody
    • CDN1 antibody
    • Cell death inhibitor 1 antibody
    • MGC117255 antibody
    • MGC3887 antibody
    • NBak antibody
    • Pro apoptotic protein BAK antibody
    see all

Images

  • All lanes : Anti-Bak antibody [NO.48A10] (ab238515) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : AGS cell extract

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 23 kDa



    Blocking buffer: 3% non-fat dry milk in TBST.

  • Paraffin-embedded mouse lung tissue stained for Bak using ab238515 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human lung tissue stained for Bak using ab238515 at 1/100 dilution in immunohistochemical analysis.

  • CRC cells stained for Bak (green) using ab238515 at 1/50 dilution in ICC/IF.

References

ab238515 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab238515.
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