Anti-BAMBI/NMA antibody [OTI5D5] (ab236499)
Key features and details
- Mouse monoclonal [OTI5D5] to BAMBI/NMA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BAMBI/NMA antibody [OTI5D5]
See all BAMBI/NMA primary antibodies -
Description
Mouse monoclonal [OTI5D5] to BAMBI/NMA -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep -
Immunogen
Recombinant fragment corresponding to Human BAMBI/NMA aa 1-200. Produced in E.coli (NP_036474).
Database link: Q13145 -
Positive control
- WB: pCMV6-ENTRY BAMBI/NMA cDNA transfected HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5D5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 29 kDa.
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Notes |
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WB
1/2000. Predicted molecular weight: 29 kDa. |
Target
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Function
Negatively regulates TGF-beta signaling. -
Tissue specificity
High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. -
Sequence similarities
Belongs to the BAMBI family. -
Developmental stage
Expression in poorly metastatic human melanoma cell lines; no expression in highly metastatic human melanoma cell lines. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25805 Human
- Entrez Gene: 68010 Mouse
- Entrez Gene: 83837 Rat
- Entrez Gene: 443242 Sheep
- Omim: 604444 Human
- SwissProt: Q13145 Human
- SwissProt: Q9D0L6 Mouse
- SwissProt: Q91XN4 Rat
see all -
Alternative names
- BAMBI antibody
- BAMBI_HUMAN antibody
- BMP and activin membrane bound inhibitor homolog antibody
see all
Images
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All lanes : Anti-BAMBI/NMA antibody [OTI5D5] (ab236499) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY BAMBI/NMA cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 29 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236499 has not yet been referenced specifically in any publications.