Overview

  • Product name

  • Description

    Rabbit polyclonal to Band 3/AE 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Band 3/AE 1 (N terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Human placenta tissue
  • General notes

    Previously labelled as Band 3. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 102 kDa).
ELISA Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
  • Tissue specificity

    Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
  • Involvement in disease

    Ovalocytosis, Southeast Asian
    Spherocytosis 4
    Renal tubular acidosis, distal, autosomal dominant
    Renal tubular acidosis, distal, with hemolytic anemia
    Renal tubular acidosis, distal, with normal red cell morphology
    Cryohydrocytosis
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
    Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    N-glycosylated.
  • Cellular localization

    Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
  • Information by UniProt
  • Database links

  • Alternative names

    • AE 1 antibody
    • AE1 antibody
    • Anion exchange protein 1 antibody
    • Anion exchanger 1 antibody
    • B3AT_HUMAN antibody
    • Band 3 anion transport protein antibody
    • Band 3 antibody
    • BND3 antibody
    • CD233 antibody
    • DI antibody
    • Diego blood group antibody
    • EMPB3 antibody
    • EPB3 antibody
    • Erythrocyte membrane protein band 3 antibody
    • Erythroid anion exchange protein antibody
    • FR antibody
    • Froese blood group antibody
    • RTA1A antibody
    • SLC4A1 antibody
    • Solute carrier family 4 anion exchanger member 1 antibody
    • Solute carrier family 4 member 1 antibody
    • SW antibody
    • Swann blood group antibody
    • Waldner blood group antibody
    • WD antibody
    • WD1 antibody
    • WR antibody
    • Wright blood group antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Band 3/AE 1 with ab78067 at 10 µg/ml.

  • All lanes : Anti-Band 3/AE 1 antibody (ab78067) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

References

This product has been referenced in:

  • Rivera-Santiago R  et al. Full-Length Anion Exchanger 1 Structure and Interactions with Ankyrin-1 Determined by Zero Length Crosslinking of Erythrocyte Membranes. Structure 25:132-145 (2017). Read more (PubMed: 27989623) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us. I am sorry for the confusion. Please select according to the following options.

If the antibody was used in Immunofluorescence on cells then select ICC/IF.

If on frozen tissue then select IHC-Fr

If used on paraffin embedded tissues then select IHC-P

If used in paraformaldehyde perfused and frozen sections then select IHC-FoFR.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

Your customer number is XXXXXXXX.

Please use the same email address to retrieve the password by clicking forget password.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question
Answer

Thank you for your email.

You need to write the discount code when submitting the Abreview in additional notes box. Please buy the antibody without any hesitation.

Best of luck for your research.

Read More

Answer

Thank you for accepting my offer.

Unfortunately you would need the discount code before the purchase. Could you tell me which antibody you would like to buy?

Read More

Answer

Thank you for contacting us.

The macaca Band 3 protein is about 94% homologous to the human protein. It seems that the anti human Band 3 antibodies will cross react with Macaca proteins also.

You can choose any of the anti Band 3 antibodies from the website. Please be advised that Macaca is not a tested species so we could not 100% confirm the x-reactivity. The use of this antibody with macaca is not guaranteed however we are happy to provide the Abreview 100% testing discount if you are interested in using any of these antibody with Macaca mulata species. Please click the link for more info.

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Let me know if you are interested in my offer. I will be happy sending you the code.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you very much for your phone call.

We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytoplasmic domain of the protein.

As discussed please send me the sequence link of Rhesus Band 3 protein, I will then do the further search.

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up