Key features and details
- Rabbit polyclonal to Band 3/AE 1
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Band 3/AE 1 antibody
See all Band 3/AE 1 primary antibodies
DescriptionRabbit polyclonal to Band 3/AE 1
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Band 3/AE 1 (N terminal) conjugated to keyhole limpet haemocyanin.
- Human placenta tissue
Previously labelled as Band 3.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab78067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 102 kDa).|
|ELISA||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
Tissue specificityDetected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
Involvement in diseaseOvalocytosis, Southeast Asian
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
modificationsPhosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
Cellular localizationCell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
- Information by UniProt
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Band 3/AE 1 with ab78067 at 10 µg/ml.
All lanes : Anti-Band 3/AE 1 antibody (ab78067) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
ab78067 has been referenced in 1 publication.
- Rivera-Santiago R et al. Full-Length Anion Exchanger 1 Structure and Interactions with Ankyrin-1 Determined by Zero Length Crosslinking of Erythrocyte Membranes. Structure 25:132-145 (2017). PubMed: 27989623