Recombinant
RabMAb

Recombinant Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)

Overview

  • Product name

    Anti-Band 3/AE 1 antibody [EPR1425(2)]
    See all Band 3/AE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1425(2)] to Band 3/AE 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human Band 3/AE 1 aa 1-100 (phospho S29) (Cysteine residue). The exact sequence is proprietary.
    Database link: P02730

  • Positive control

    • Human red blood cell lysate; Human lung and spleen tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Band 3.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1425(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000 - 1/500000. Predicted molecular weight: 100 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • Tissue specificity

      Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • Involvement in disease

      Ovalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
      Cryohydrocytosis
    • Sequence similarities

      Belongs to the anion exchanger (TC 2.A.31) family.
    • Post-translational
      modifications

      Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
      N-glycosylated.
    • Cellular localization

      Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • Database links

    • Alternative names

      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • Band 3 antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Band 3/AE 1 at 1/250 dilution.  Note erythrocyte staining.

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Band 3/AE 1 wtih ab175214 at 1/250 dilution. Note erythrocyte staining.

    • Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214) at 1/100000 dilution + Human red blood cell lysate at 10 µg

      Predicted band size: 100 kDa

    References

    ab175214 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175214.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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