Product nameAnti-Band 3/AE 1 antibody [EPR1425(2)]
See all Band 3/AE 1 primary antibodies
DescriptionRabbit monoclonal [EPR1425(2)] to Band 3/AE 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
within Human Band 3/AE 1 aa 1-100 (phospho S29) (Cysteine residue). The exact sequence is proprietary.
Database link: P02730
- Human red blood cell lysate; Human lung and spleen tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Band 3.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100000 - 1/500000. Predicted molecular weight: 100 kDa.|
|IHC-P||1/100 - 1/250.|
FunctionFunctions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
Tissue specificityDetected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
Involvement in diseaseOvalocytosis, Southeast Asian
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
modificationsPhosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
Cellular localizationCell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
- Information by UniProt
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Band 3/AE 1 at 1/250 dilution. Note erythrocyte staining.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Band 3/AE 1 wtih ab175214 at 1/250 dilution. Note erythrocyte staining.
Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214) at 1/100000 dilution + Human red blood cell lysate at 10 µg
Predicted band size: 100 kDa
ab175214 has not yet been referenced specifically in any publications.